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FLEXIQinase, a mass spectrometry-based assay, to unveil multi-kinase mechanisms
We introduce a mass spectrometry-based method that provides residue-resolved quantitative information about protein phosphorylation. In this FLEXIQinase assay we combined our Full-Length Expressed Stable Isotope-labeled Protein for Quantification strategy (FLEXIQuant) with a traditional kinase assay...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3595540/ https://www.ncbi.nlm.nih.gov/pubmed/22484849 http://dx.doi.org/10.1038/nmeth.1970 |