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Breaking the Diffraction Barrier Using Fluorescence Emission Difference Microscopy
We propose a novel physical mechanism for breaking the diffraction barrier in the far field. Termed fluorescence emission difference microscopy (FED), our approach is based on the intensity difference between two differently acquired images. When fluorescence saturation is applied, the resolving abi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3595927/ https://www.ncbi.nlm.nih.gov/pubmed/23486546 http://dx.doi.org/10.1038/srep01441 |