Construction and identification of recombinant lentiviral vector containing HIV-1 Tat gene and its expression in 293T cells()

OBJECTIVE: To construct a lentiviral vector expressing HIV-1 Tat and identify its expression in 293T cells. METHODS: The gene fragment of HIV-1 Tat(101) was subcloned to lentiviral transfer vector pHAGE-CMV-MCS-IZsGreen, which was named pHAGE-Tat. Then the constructed pHAGE-Tat was used to co-transf...

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Detalles Bibliográficos
Autores principales: Wei, Bingbing, Feng, Ninghan, Zhou, Feng, Lu, Chun, Su, Jiantang, Hua, Lixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial Department of Journal of Biomedical Research 2010
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596536/
https://www.ncbi.nlm.nih.gov/pubmed/23554612
http://dx.doi.org/10.1016/S1674-8301(10)60009-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596536/
https://www.ncbi.nlm.nih.gov/pubmed/23554612
http://dx.doi.org/10.1016/S1674-8301(10)60009-7

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