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Construction and identification of recombinant lentiviral vector containing HIV-1 Tat gene and its expression in 293T cells()
OBJECTIVE: To construct a lentiviral vector expressing HIV-1 Tat and identify its expression in 293T cells. METHODS: The gene fragment of HIV-1 Tat(101) was subcloned to lentiviral transfer vector pHAGE-CMV-MCS-IZsGreen, which was named pHAGE-Tat. Then the constructed pHAGE-Tat was used to co-transf...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Editorial Department of Journal of Biomedical Research
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596536/ https://www.ncbi.nlm.nih.gov/pubmed/23554612 http://dx.doi.org/10.1016/S1674-8301(10)60009-7 |