Purification and characterization of nitrile hydratase of mutant 4D of Rhodococcus rhodochrous PA-34

Nitrile hydratase (NHase; E.C. 4.2.1.84) has been purified and characterized using ammonium sulfate precipitation, ion exchange chromatography and gel filtration chromatography from the mutant 4D of Rhodococcus rhodochrous PA-34. The SDS-PAGE and MALDI-TOF analysis of the purified enzyme revealed th...

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Detalles Bibliográficos
Autores principales: Pratush, Amit, Seth, Amit, Bhalla, Tek Chand
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2012
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597139/
https://www.ncbi.nlm.nih.gov/pubmed/28324571
http://dx.doi.org/10.1007/s13205-012-0081-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597139/
https://www.ncbi.nlm.nih.gov/pubmed/28324571
http://dx.doi.org/10.1007/s13205-012-0081-5

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