Cargando…

Purification and characterization of nitrile hydratase of mutant 4D of Rhodococcus rhodochrous PA-34

Nitrile hydratase (NHase; E.C. 4.2.1.84) has been purified and characterized using ammonium sulfate precipitation, ion exchange chromatography and gel filtration chromatography from the mutant 4D of Rhodococcus rhodochrous PA-34. The SDS-PAGE and MALDI-TOF analysis of the purified enzyme revealed th...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pratush, Amit, Seth, Amit, Bhalla, Tek Chand
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2012
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597139/ https://www.ncbi.nlm.nih.gov/pubmed/28324571 http://dx.doi.org/10.1007/s13205-012-0081-5

Ejemplares similares

Overexpression and characterization of two types of nitrile hydratases from Rhodococcus rhodochrous J1
por: Lan, Yao, et al.
Publicado: (2017)

Substrate Profiling of the Cobalt Nitrile Hydratase from Rhodococcus rhodochrous ATCC BAA 870
por: Mashweu, Adelaide R., et al.
Publicado: (2020)

Response surface methodology: An effective optimization strategy for enhanced production of nitrile hydratase (NHase) by Rhodococcus rhodochrous (RS-6)
por: Sahu, Ruchi, et al.
Publicado: (2020)

Nitrile hydratase of Rhodococcus erythropolis: characterization of the enzyme and the use of whole cells for biotransformation of nitriles
por: Kamble, Ashwini L., et al.
Publicado: (2012)

Biotransformation of (–)-Isopulegol by Rhodococcus rhodochrous
por: Ivshina, Irina B., et al.
Publicado: (2022)

Simultaneous purification of nitrile hydratase and amidase of Alcaligenes sp. MTCC 10674
por: Bhatia, S. K., et al.
Publicado: (2013)

An amino acid at position 142 in nitrilase from Rhodococcus rhodochrous ATCC 33278 determines the substrate specificity for aliphatic and aromatic nitriles
por: Yeom, Soo-Jin, et al.
Publicado: (2008)

Draft Genome Sequence of Rhodococcus rhodochrous Strain ATCC 21198
por: Shields-Menard, Sara A., et al.
Publicado: (2014)

Draft genome sequence of Rhodococcus rhodochrous strain ATCC 17895
por: Chen, Bi-Shuang, et al.
Publicado: (2013)

Regio- and stereoselective oxidation of unactivated C–H bonds with Rhodococcus rhodochrous
por: O’Reilly, Elaine, et al.
Publicado: (2012)

The complete genome sequence of the nitrile biocatalyst Rhodocccus rhodochrous ATCC BAA-870
por: Frederick, Joni, et al.
Publicado: (2020)

A Nitrile Hydratase in the Eukaryote Monosiga brevicollis
por: Foerstner, Konrad U., et al.
Publicado: (2008)

High Regioselectivity Production of 5-Cyanovaleramide from Adiponitrile by a Novel Nitrile Hydratase Derived from Rhodococcus erythropolis CCM2595
por: Wang, Li, et al.
Publicado: (2020)

Draft Genome of Rhodococcus rhodochrous TRN7, Isolated from the Coast of Trindade Island, Brazil
por: Rodrigues, Edmo M., et al.
Publicado: (2016)

Efficient biodegradation of chlorophenols in aqueous phase by magnetically immobilized aniline-degrading Rhodococcus rhodochrous strain
por: Hou, Jianfeng, et al.
Publicado: (2016)

Rhodococcus rhodochrous IEGM 1360, an Effective Biocatalyst of C3 Oxidative Transformation of Oleanane Triterpenoids
por: Luchnikova, N. A., et al.
Publicado: (2023)

Adaptive laboratory evolution of Rhodococcus rhodochrous DSM6263 for chlorophenol degradation under hypersaline condition
por: Zheng, Jie, et al.
Publicado: (2023)

Nitrile Hydratase Genes Are Present in Multiple Eukaryotic Supergroups
por: Marron, Alan O., et al.
Publicado: (2012)

Engineering enhanced thermostability into the Geobacillus pallidus nitrile hydratase
por: Van Wyk, Jennifer C., et al.
Publicado: (2022)

Functional Expression and Characterization of a Panel of Cobalt and Iron-Dependent Nitrile Hydratases
por: Grill, Birgit, et al.
Publicado: (2020)

A preliminary report on the contact-independent antagonism of Pseudogymnoascus destructans by Rhodococcus rhodochrous strain DAP96253
por: Cornelison, Christopher T, et al.
Publicado: (2014)

Biodegradation of flonicamid by Ensifer adhaerens CGMCC 6315 and enzymatic characterization of the nitrile hydratases involved
por: Zhao, Yun-Xiu, et al.
Publicado: (2021)

Draft Genome Sequence of Rhodococcus rhodochrous Strain G38GP, Isolated from the Madagascar Hissing Cockroach
por: Guzman, Juan, et al.
Publicado: (2021)

Recent Advances and Promises in Nitrile Hydratase: From Mechanism to Industrial Applications
por: Cheng, Zhongyi, et al.
Publicado: (2020)

Identification of the two-component guaiacol demethylase system from Rhodococcus rhodochrous and expression in Pseudomonas putida EM42 for guaiacol assimilation
por: García-Hidalgo, Javier, et al.
Publicado: (2019)

Data on genome sequencing, assembly, annotation and genomic analysis of Rhodococcus rhodochrous strain SPC17 isolated from Lonar Lake
por: Kumar, Satish, et al.
Publicado: (2020)

The Alpha Subunit of Nitrile Hydratase Is Sufficient for Catalytic Activity and Post-Translational Modification
por: Nelp, Micah T., et al.
Publicado: (2014)

Self-Subunit Swapping Occurs in Another Gene Type of Cobalt Nitrile Hydratase
por: Liu, Yi, et al.
Publicado: (2012)

A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
por: Angelini, Leticia Mara Lima, et al.
Publicado: (2015)

Insight into the broadened substrate scope of nitrile hydratase by static and dynamic structure analysis
por: Ma, Dong, et al.
Publicado: (2022)

Purification, characterization, and cloning of a bifunctional molybdoenzyme with hydratase and alcohol dehydrogenase activity
por: Jin, Jianfeng, et al.
Publicado: (2010)

Recombinant expression, purification and biochemical characterization of kievitone hydratase from Nectria haematococca
por: Engleder, Matthias, et al.
Publicado: (2018)

The Active Site Sulfenic Acid Ligand in Nitrile Hydratases Can Function as a Nucleophile
por: Martinez, Salette, et al.
Publicado: (2014)

An Aeroplysinin-1 Specific Nitrile Hydratase Isolated from the Marine Sponge Aplysina cavernicola
por: Lipowicz, Bartosz, et al.
Publicado: (2013)

Computational Design of Nitrile Hydratase from Pseudonocardia thermophila JCM3095 for Improved Thermostability
por: Cheng, Zhongyi, et al.
Publicado: (2020)

Unexpected NADPH Hydratase Activity in the Nitrile Reductase QueF from Escherichia coli
por: Jung, Jihye, et al.
Publicado: (2020)

Exploring the abundance of oleate hydratases in the genus Rhodococcus—discovery of novel enzymes with complementary substrate scope
por: Busch, Hanna, et al.
Publicado: (2020)

Draft Genome Sequence of Rhodococcus rhodochrous Strain KG-21, a Soil Isolate from Oil Fields of Krishna-Godavari Basin, India
por: Dawar, Chhavi, et al.
Publicado: (2015)

Enhanced bioremediation of triclocarban-contaminated soil by Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 immobilized on biochar and microbial community response
por: Miao, Lei, et al.
Publicado: (2023)

Biochemical and spectroscopic characterization of purified Latex Clearing Protein (Lcp) from newly isolated rubber degrading Rhodococcus rhodochrous strain RPK1 reveals novel properties of Lcp
por: Watcharakul, Sirimaporn, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_rm5uidqu9knq4b1lcbgst0i685