An efficient method for genome-wide polyadenylation site mapping and RNA quantification

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The use of alternative poly(A) sites is common and affects the post-transcriptional fate of mRNA, including its stability, subcellular localization and translation. Here, we present a method to identify poly(A) sites in a genome-wide and strand-specific manner. This method, termed 3′T-fill, initiall...

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Detalles Bibliográficos
Autores principales: Wilkening, Stefan, Pelechano, Vicent, Järvelin, Aino I., Tekkedil, Manu M., Anders, Simon, Benes, Vladimir, Steinmetz, Lars M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597643/
https://www.ncbi.nlm.nih.gov/pubmed/23295673
http://dx.doi.org/10.1093/nar/gks1249

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597643/
https://www.ncbi.nlm.nih.gov/pubmed/23295673
http://dx.doi.org/10.1093/nar/gks1249

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