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Careful Selection of Reference Genes Is Required for Reliable Performance of RT-qPCR in Human Normal and Cancer Cell Lines

Reverse Transcription - quantitative Polymerase Chain Reaction (RT-qPCR) is a standard technique in most laboratories. The selection of reference genes is essential for data normalization and the selection of suitable reference genes remains critical. Our aim was to 1) review the literature since im...

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Detalles Bibliográficos
Autores principales: Jacob, Francis, Guertler, Rea, Naim, Stephanie, Nixdorf, Sheri, Fedier, André, Hacker, Neville F., Heinzelmann-Schwarz, Viola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3598660/
https://www.ncbi.nlm.nih.gov/pubmed/23554992
http://dx.doi.org/10.1371/journal.pone.0059180