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Careful Selection of Reference Genes Is Required for Reliable Performance of RT-qPCR in Human Normal and Cancer Cell Lines
Reverse Transcription - quantitative Polymerase Chain Reaction (RT-qPCR) is a standard technique in most laboratories. The selection of reference genes is essential for data normalization and the selection of suitable reference genes remains critical. Our aim was to 1) review the literature since im...
Autores principales: | Jacob, Francis, Guertler, Rea, Naim, Stephanie, Nixdorf, Sheri, Fedier, André, Hacker, Neville F., Heinzelmann-Schwarz, Viola |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3598660/ https://www.ncbi.nlm.nih.gov/pubmed/23554992 http://dx.doi.org/10.1371/journal.pone.0059180 |
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