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Expression of a secretory α-glucosidase II from Apis cerana indica in Pichia pastoris and its characterization

BACKGROUND: α–glucosidase (HBGase) plays a key role in hydrolyzing α-glucosidic linkages. In Apis mellifera, three isoforms of HBGase (I, II and III) have been reported, which differ in their nucleotide composition, encoding amino acid sequences and enzyme kinetics. Recombinant (r)HBGase II from A....

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Detalles Bibliográficos
Autores principales: Kaewmuangmoon, Jirattikarn, Kilaso, Manlika, Leartsakulpanich, Ubolsree, Kimura, Kiyoshi, Kimura, Atsuo, Chanchao, Chanpen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599193/
https://www.ncbi.nlm.nih.gov/pubmed/23419073
http://dx.doi.org/10.1186/1472-6750-13-16