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Evaluation of reference genes for reverse transcription quantitative PCR analyses of fish-pathogenic Francisella strains exposed to different growth conditions

BACKGROUND: Reverse transcription quantitative PCR has become a powerful technique to monitor mRNA transcription in response to different environmental conditions in many bacterial species. However, correct evaluation of data requires accurate and reliable use of reference genes whose transcription...

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Detalles Bibliográficos
Autores principales: Brudal, Espen, Winther-Larsen, Hanne Cecilie, Colquhoun, Duncan John, Duodu, Samuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599356/
https://www.ncbi.nlm.nih.gov/pubmed/23452832
http://dx.doi.org/10.1186/1756-0500-6-76