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Evaluation of reference genes for reverse transcription quantitative PCR analyses of fish-pathogenic Francisella strains exposed to different growth conditions
BACKGROUND: Reverse transcription quantitative PCR has become a powerful technique to monitor mRNA transcription in response to different environmental conditions in many bacterial species. However, correct evaluation of data requires accurate and reliable use of reference genes whose transcription...
Autores principales: | Brudal, Espen, Winther-Larsen, Hanne Cecilie, Colquhoun, Duncan John, Duodu, Samuel |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599356/ https://www.ncbi.nlm.nih.gov/pubmed/23452832 http://dx.doi.org/10.1186/1756-0500-6-76 |
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