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DNA extract characterization process for microbial detection methods development and validation

BACKGROUND: Quantitative polymerase chain reaction (qPCR) assays used in pathogen detection require rigorous methods development including characterizing DNA extraction products. A DNA extract characterization process is demonstrated using DNA extracted from five different cells types (two Gram-nega...

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Detalles Bibliográficos
Autores principales: Olson, Nathan D, Morrow, Jayne B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599793/
https://www.ncbi.nlm.nih.gov/pubmed/23206278
http://dx.doi.org/10.1186/1756-0500-5-668