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DNA extract characterization process for microbial detection methods development and validation
BACKGROUND: Quantitative polymerase chain reaction (qPCR) assays used in pathogen detection require rigorous methods development including characterizing DNA extraction products. A DNA extract characterization process is demonstrated using DNA extracted from five different cells types (two Gram-nega...
Autores principales: | Olson, Nathan D, Morrow, Jayne B |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3599793/ https://www.ncbi.nlm.nih.gov/pubmed/23206278 http://dx.doi.org/10.1186/1756-0500-5-668 |
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