High-level over-expression, purification, and crystallization of a novel phospholipase C/sphingomyelinase from Pseudomonas aeruginosa

The hemolytic phospholipase C/sphingomyelinase PlcH from the opportunistic pathogen Pseudomonas aeruginosa represents the founding member of a growing family of virulence factors identified in a wide range of bacterial and fungal pathogens. In P. aeruginosa PlcH is co-expressed with a 17 kDa chapero...

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Detalles Bibliográficos
Autores principales: Truan, Daphné, Vasil, Adriana, Stonehouse, Martin, Vasil, Michael L., Pohl, Ehmke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601568/
https://www.ncbi.nlm.nih.gov/pubmed/23201280
http://dx.doi.org/10.1016/j.pep.2012.11.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601568/
https://www.ncbi.nlm.nih.gov/pubmed/23201280
http://dx.doi.org/10.1016/j.pep.2012.11.005

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