Production of soluble eukaryotic recombinant proteins in E. coli is favoured in early log-phase cultures induced at low temperature

BACKGROUND: Producing recombinant plant proteins expressed in Escherichia coli produce in high yields and in a soluble and functional form can be difficult. Under overexpression conditions, proteins frequently accumulate as insoluble aggregates (inclusion bodies) within the producing bacteria. We ev...

Descripción completa

Detalles Bibliográficos
Autores principales: San-Miguel, Teresa, Pérez-Bermúdez, Pedro, Gavidia, Isabel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing AG 2013
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3602615/
https://www.ncbi.nlm.nih.gov/pubmed/23525091
http://dx.doi.org/10.1186/2193-1801-2-89

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3602615/
https://www.ncbi.nlm.nih.gov/pubmed/23525091
http://dx.doi.org/10.1186/2193-1801-2-89

Ejemplares similares