Effective RNAi-mediated β2-microglobulin loss of function by transgenesis in Xenopus laevis

To impair MHC class I (class I) function in vivo in the amphibian Xenopus, we developed an effective reverse genetic loss of function approach by combining I-SceI meganuclease-mediated transgenesis with RNAi technology. We generated transgenic outbred X. laevis and isogenetic laevis/gilli cloned lin...

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Detalles Bibliográficos
Autores principales: Nedelkovska, Hristina, Edholm, Eva-Stina, Haynes, Nikesha, Robert, Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3603415/
https://www.ncbi.nlm.nih.gov/pubmed/23519478
http://dx.doi.org/10.1242/bio.20133483

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3603415/
https://www.ncbi.nlm.nih.gov/pubmed/23519478
http://dx.doi.org/10.1242/bio.20133483

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