Chemerin Is an Antimicrobial Agent in Human Epidermis

Chemerin, a chemoattractant ligand for chemokine-like receptor 1 (CMKLR1) is predicted to share similar tertiary structure with antibacterial cathelicidins. Recombinant chemerin has antimicrobial activity. Here we show that endogenous chemerin is abundant in human epidermis, and that inhibition of b...

Descripción completa

Detalles Bibliográficos
Autores principales: Banas, Magdalena, Zabieglo, Katarzyna, Kasetty, Gopinath, Kapinska-Mrowiecka, Monika, Borowczyk, Julia, Drukala, Justyna, Murzyn, Krzysztof, Zabel, Brian A., Butcher, Eugene C., Schroeder, Jens M., Schmidtchen, Artur, Cichy, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3604073/
https://www.ncbi.nlm.nih.gov/pubmed/23527010
http://dx.doi.org/10.1371/journal.pone.0058709
_version_ 1782263740180201472
author Banas, Magdalena
Zabieglo, Katarzyna
Kasetty, Gopinath
Kapinska-Mrowiecka, Monika
Borowczyk, Julia
Drukala, Justyna
Murzyn, Krzysztof
Zabel, Brian A.
Butcher, Eugene C.
Schroeder, Jens M.
Schmidtchen, Artur
Cichy, Joanna
author_facet Banas, Magdalena
Zabieglo, Katarzyna
Kasetty, Gopinath
Kapinska-Mrowiecka, Monika
Borowczyk, Julia
Drukala, Justyna
Murzyn, Krzysztof
Zabel, Brian A.
Butcher, Eugene C.
Schroeder, Jens M.
Schmidtchen, Artur
Cichy, Joanna
author_sort Banas, Magdalena
collection PubMed
description Chemerin, a chemoattractant ligand for chemokine-like receptor 1 (CMKLR1) is predicted to share similar tertiary structure with antibacterial cathelicidins. Recombinant chemerin has antimicrobial activity. Here we show that endogenous chemerin is abundant in human epidermis, and that inhibition of bacteria growth by exudates from organ cultures of primary human skin keratinocytes is largely chemerin-dependent. Using a panel of overlapping chemerin-derived synthetic peptides, we demonstrate that the antibacterial activity of chemerin is primarily mediated by Val(66)-Pro(85), which causes direct bacterial lysis. Therefore, chemerin is an antimicrobial agent in human skin.
format Online
Article
Text
id pubmed-3604073
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-36040732013-03-22 Chemerin Is an Antimicrobial Agent in Human Epidermis Banas, Magdalena Zabieglo, Katarzyna Kasetty, Gopinath Kapinska-Mrowiecka, Monika Borowczyk, Julia Drukala, Justyna Murzyn, Krzysztof Zabel, Brian A. Butcher, Eugene C. Schroeder, Jens M. Schmidtchen, Artur Cichy, Joanna PLoS One Research Article Chemerin, a chemoattractant ligand for chemokine-like receptor 1 (CMKLR1) is predicted to share similar tertiary structure with antibacterial cathelicidins. Recombinant chemerin has antimicrobial activity. Here we show that endogenous chemerin is abundant in human epidermis, and that inhibition of bacteria growth by exudates from organ cultures of primary human skin keratinocytes is largely chemerin-dependent. Using a panel of overlapping chemerin-derived synthetic peptides, we demonstrate that the antibacterial activity of chemerin is primarily mediated by Val(66)-Pro(85), which causes direct bacterial lysis. Therefore, chemerin is an antimicrobial agent in human skin. Public Library of Science 2013-03-20 /pmc/articles/PMC3604073/ /pubmed/23527010 http://dx.doi.org/10.1371/journal.pone.0058709 Text en © 2013 Banas et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Banas, Magdalena
Zabieglo, Katarzyna
Kasetty, Gopinath
Kapinska-Mrowiecka, Monika
Borowczyk, Julia
Drukala, Justyna
Murzyn, Krzysztof
Zabel, Brian A.
Butcher, Eugene C.
Schroeder, Jens M.
Schmidtchen, Artur
Cichy, Joanna
Chemerin Is an Antimicrobial Agent in Human Epidermis
title Chemerin Is an Antimicrobial Agent in Human Epidermis
title_full Chemerin Is an Antimicrobial Agent in Human Epidermis
title_fullStr Chemerin Is an Antimicrobial Agent in Human Epidermis
title_full_unstemmed Chemerin Is an Antimicrobial Agent in Human Epidermis
title_short Chemerin Is an Antimicrobial Agent in Human Epidermis
title_sort chemerin is an antimicrobial agent in human epidermis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3604073/
https://www.ncbi.nlm.nih.gov/pubmed/23527010
http://dx.doi.org/10.1371/journal.pone.0058709
work_keys_str_mv AT banasmagdalena chemerinisanantimicrobialagentinhumanepidermis
AT zabieglokatarzyna chemerinisanantimicrobialagentinhumanepidermis
AT kasettygopinath chemerinisanantimicrobialagentinhumanepidermis
AT kapinskamrowieckamonika chemerinisanantimicrobialagentinhumanepidermis
AT borowczykjulia chemerinisanantimicrobialagentinhumanepidermis
AT drukalajustyna chemerinisanantimicrobialagentinhumanepidermis
AT murzynkrzysztof chemerinisanantimicrobialagentinhumanepidermis
AT zabelbriana chemerinisanantimicrobialagentinhumanepidermis
AT butchereugenec chemerinisanantimicrobialagentinhumanepidermis
AT schroederjensm chemerinisanantimicrobialagentinhumanepidermis
AT schmidtchenartur chemerinisanantimicrobialagentinhumanepidermis
AT cichyjoanna chemerinisanantimicrobialagentinhumanepidermis

Ejemplares similares