Comparison of a quantitative real-time polymerase chain reaction (qPCR) with conventional PCR, bacterial culture and ELISA for detection of Mycobacterium avium subsp. paratuberculosis infection in sheep showing pathology of Johne’s disease

A quantitative real-time PCR (qPCR) assay employing IS900 gene specific primers of Mycobacterium avium subsp. parartuberculosis (MAP) was compared with conventional PCR, bacterial culture and enzyme-linked immunosorbent assay in 38 sheep showing granulomatous enteritis and lymphadenitis with and wit...

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Detalles Bibliográficos
Autores principales: Sonawane, Ganesh G, Tripathi, Bhupendra N
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing AG 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3604594/
https://www.ncbi.nlm.nih.gov/pubmed/23539663
http://dx.doi.org/10.1186/2193-1801-2-45

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3604594/
https://www.ncbi.nlm.nih.gov/pubmed/23539663
http://dx.doi.org/10.1186/2193-1801-2-45

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