Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

BACKGROUND: In Escherichia coli many heterologous proteins are produced in the periplasm. To direct these proteins to the periplasm, they are equipped with an N-terminal signal sequence so that they can traverse the cytoplasmic membrane via the protein-conducting Sec-translocon. For poorly understoo...

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Detalles Bibliográficos
Autores principales: Schlegel, Susan, Rujas, Edurne, Ytterberg, Anders Jimmy, Zubarev, Roman A, Luirink, Joen, de Gier, Jan-Willem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605120/
https://www.ncbi.nlm.nih.gov/pubmed/23497240
http://dx.doi.org/10.1186/1475-2859-12-24

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605120/
https://www.ncbi.nlm.nih.gov/pubmed/23497240
http://dx.doi.org/10.1186/1475-2859-12-24

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