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Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption

BACKGROUND: The cloning of gene sequences forms the basis for many molecular biological studies. One important step in the cloning process is the isolation of bacterial transformants carrying vector DNA. This involves a vector-encoded selectable marker gene, which in most cases, confers resistance t...

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Detalles Bibliográficos
Autores principales: Manna, Sam, Harman, Ashley, Accari, Jessica, Barth, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605328/
https://www.ncbi.nlm.nih.gov/pubmed/23497512
http://dx.doi.org/10.1186/1756-0500-6-85