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Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption
BACKGROUND: The cloning of gene sequences forms the basis for many molecular biological studies. One important step in the cloning process is the isolation of bacterial transformants carrying vector DNA. This involves a vector-encoded selectable marker gene, which in most cases, confers resistance t...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605328/ https://www.ncbi.nlm.nih.gov/pubmed/23497512 http://dx.doi.org/10.1186/1756-0500-6-85 |