A mutant screening method by critical annealing temperature-PCR for site-directed mutagenesis

BACKGROUND: Distinguishing desired mutants from parental templates and undesired mutants is a problem not well solved in Quikchange™ mutagenesis. Although Dpn I digestion can eliminate methylated parental (WT) DNA, the efficiency is not satisfying due to the existence of hemi-methylated DNA in the P...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Ying, Wu, Ting, Song, Jian, Chen, Xuelian, Zhang, Yu, Wan, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606131/
https://www.ncbi.nlm.nih.gov/pubmed/23497028
http://dx.doi.org/10.1186/1472-6750-13-21

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606131/
https://www.ncbi.nlm.nih.gov/pubmed/23497028
http://dx.doi.org/10.1186/1472-6750-13-21

Ejemplares similares