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A mutant screening method by critical annealing temperature-PCR for site-directed mutagenesis

BACKGROUND: Distinguishing desired mutants from parental templates and undesired mutants is a problem not well solved in Quikchange™ mutagenesis. Although Dpn I digestion can eliminate methylated parental (WT) DNA, the efficiency is not satisfying due to the existence of hemi-methylated DNA in the P...

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Detalles Bibliográficos
Autores principales:	Liu, Ying, Wu, Ting, Song, Jian, Chen, Xuelian, Zhang, Yu, Wan, Yu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606131/ https://www.ncbi.nlm.nih.gov/pubmed/23497028 http://dx.doi.org/10.1186/1472-6750-13-21

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