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A library of programmable DNAzymes that operate in a cellular environment
DNAzymes were used as inhibitory agents in a variety of experimental disease settings, such as cancer, viral infections and even HIV. Drugs that become active only upon the presence of preprogrammed abnormal environmental conditions may enable selective molecular therapy by targeting abnormal cells...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607177/ https://www.ncbi.nlm.nih.gov/pubmed/23525068 http://dx.doi.org/10.1038/srep01535 |
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author | Kahan-Hanum, Maya Douek, Yehonatan Adar, Rivka Shapiro, Ehud |
author_facet | Kahan-Hanum, Maya Douek, Yehonatan Adar, Rivka Shapiro, Ehud |
author_sort | Kahan-Hanum, Maya |
collection | PubMed |
description | DNAzymes were used as inhibitory agents in a variety of experimental disease settings, such as cancer, viral infections and even HIV. Drugs that become active only upon the presence of preprogrammed abnormal environmental conditions may enable selective molecular therapy by targeting abnormal cells without injuring normal cells. Here we show a novel programmable DNAzyme library composed of variety of Boolean logic gates, including YES, AND, NOT, OR, NAND, ANDNOT, XOR, NOR and 3-input-AND gate, that uses both miRNAs and mRNAs as inputs. Each gate is based on the c-jun cleaving Dz13 DNAzyme and active only in the presence of specific input combinations. The library is modular, supports arbitrary inputs and outputs, cascadable, highly specific and robust. We demonstrate the library's potential diagnostic abilities on miRNA and mRNA combinations in cell lysate and its ability to operate in a cellular environment by using beacon-like c-jun mimicking substrate in living mammalian cells. |
format | Online Article Text |
id | pubmed-3607177 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-36071772013-03-25 A library of programmable DNAzymes that operate in a cellular environment Kahan-Hanum, Maya Douek, Yehonatan Adar, Rivka Shapiro, Ehud Sci Rep Article DNAzymes were used as inhibitory agents in a variety of experimental disease settings, such as cancer, viral infections and even HIV. Drugs that become active only upon the presence of preprogrammed abnormal environmental conditions may enable selective molecular therapy by targeting abnormal cells without injuring normal cells. Here we show a novel programmable DNAzyme library composed of variety of Boolean logic gates, including YES, AND, NOT, OR, NAND, ANDNOT, XOR, NOR and 3-input-AND gate, that uses both miRNAs and mRNAs as inputs. Each gate is based on the c-jun cleaving Dz13 DNAzyme and active only in the presence of specific input combinations. The library is modular, supports arbitrary inputs and outputs, cascadable, highly specific and robust. We demonstrate the library's potential diagnostic abilities on miRNA and mRNA combinations in cell lysate and its ability to operate in a cellular environment by using beacon-like c-jun mimicking substrate in living mammalian cells. Nature Publishing Group 2013-03-25 /pmc/articles/PMC3607177/ /pubmed/23525068 http://dx.doi.org/10.1038/srep01535 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Kahan-Hanum, Maya Douek, Yehonatan Adar, Rivka Shapiro, Ehud A library of programmable DNAzymes that operate in a cellular environment |
title | A library of programmable DNAzymes that operate in a cellular environment |
title_full | A library of programmable DNAzymes that operate in a cellular environment |
title_fullStr | A library of programmable DNAzymes that operate in a cellular environment |
title_full_unstemmed | A library of programmable DNAzymes that operate in a cellular environment |
title_short | A library of programmable DNAzymes that operate in a cellular environment |
title_sort | library of programmable dnazymes that operate in a cellular environment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607177/ https://www.ncbi.nlm.nih.gov/pubmed/23525068 http://dx.doi.org/10.1038/srep01535 |
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