Repeatable Construction Method for Engineered Zinc Finger Nuclease Based on Overlap Extension PCR and TA-Cloning

Zinc finger nuclease (ZFN) is a useful tool for endogenous site-directed genome modification. The development of an easier, less expensive and repeatedly usable construction method for various sequences of ZFNs should contribute to the further widespread use of this technology. Here, we establish a...

Descripción completa

Detalles Bibliográficos
Autores principales: Fujii, Wataru, Kano, Kiyoshi, Sugiura, Koji, Naito, Kunihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607563/
https://www.ncbi.nlm.nih.gov/pubmed/23536890
http://dx.doi.org/10.1371/journal.pone.0059801

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607563/
https://www.ncbi.nlm.nih.gov/pubmed/23536890
http://dx.doi.org/10.1371/journal.pone.0059801

Ejemplares similares