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Robust Detection of Rare Species Using Environmental DNA: The Importance of Primer Specificity

Environmental DNA (eDNA) is being rapidly adopted as a tool to detect rare animals. Quantitative PCR (qPCR) using probe-based chemistries may represent a particularly powerful tool because of the method’s sensitivity, specificity, and potential to quantify target DNA. However, there has been little...

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Detalles Bibliográficos
Autores principales: Wilcox, Taylor M., McKelvey, Kevin S., Young, Michael K., Jane, Stephen F., Lowe, Winsor H., Whiteley, Andrew R., Schwartz, Michael K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608683/
https://www.ncbi.nlm.nih.gov/pubmed/23555689
http://dx.doi.org/10.1371/journal.pone.0059520