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The Tyrosine Kinase Btk Regulates the Macrophage Response to Listeria monocytogenes Infection
In this study we investigated the role of Bruton's tyrosine kinase (Btk) in the immune response to the Gram-positive intracellular bacterium Listeria monocytogenes (Lm). In response to Lm infection, Btk was activated in bone marrow-derived macrophages (BMMs) and Btk (−/−) BMMs showed enhanced T...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609758/ https://www.ncbi.nlm.nih.gov/pubmed/23544144 http://dx.doi.org/10.1371/journal.pone.0060476 |
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author | Köprülü, Afitap Derya Kastner, Renate Wienerroither, Sebastian Lassnig, Caroline Putz, Eva Maria Majer, Olivia Reutterer, Benjamin Sexl, Veronika Kuchler, Karl Müller, Mathias Decker, Thomas Ellmeier, Wilfried |
author_facet | Köprülü, Afitap Derya Kastner, Renate Wienerroither, Sebastian Lassnig, Caroline Putz, Eva Maria Majer, Olivia Reutterer, Benjamin Sexl, Veronika Kuchler, Karl Müller, Mathias Decker, Thomas Ellmeier, Wilfried |
author_sort | Köprülü, Afitap Derya |
collection | PubMed |
description | In this study we investigated the role of Bruton's tyrosine kinase (Btk) in the immune response to the Gram-positive intracellular bacterium Listeria monocytogenes (Lm). In response to Lm infection, Btk was activated in bone marrow-derived macrophages (BMMs) and Btk (−/−) BMMs showed enhanced TNF-α, IL-6 and IL-12p40 secretion, while type I interferons were produced at levels similar to wild-type (wt) BMMs. Although Btk-deficient BMMs displayed reduced phagocytosis of E. coli fragments, there was no difference between wt and Btk (−/−) BMMs in the uptake of Lm upon infection. Moreover, there was no difference in the response to heat-killed Lm between wt and Btk (−/−) BMMs, suggesting a role for Btk in signaling pathways that are induced by intracellular Lm. Finally, Btk (−/−) mice displayed enhanced resistance and an increased mean survival time upon Lm infection in comparison to wt mice. This correlated with elevated IFN-γ and IL-12p70 serum levels in Btk (−/−) mice at day 1 after infection. Taken together, our data suggest an important regulatory role for Btk in macrophages during Lm infection. |
format | Online Article Text |
id | pubmed-3609758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36097582013-03-29 The Tyrosine Kinase Btk Regulates the Macrophage Response to Listeria monocytogenes Infection Köprülü, Afitap Derya Kastner, Renate Wienerroither, Sebastian Lassnig, Caroline Putz, Eva Maria Majer, Olivia Reutterer, Benjamin Sexl, Veronika Kuchler, Karl Müller, Mathias Decker, Thomas Ellmeier, Wilfried PLoS One Research Article In this study we investigated the role of Bruton's tyrosine kinase (Btk) in the immune response to the Gram-positive intracellular bacterium Listeria monocytogenes (Lm). In response to Lm infection, Btk was activated in bone marrow-derived macrophages (BMMs) and Btk (−/−) BMMs showed enhanced TNF-α, IL-6 and IL-12p40 secretion, while type I interferons were produced at levels similar to wild-type (wt) BMMs. Although Btk-deficient BMMs displayed reduced phagocytosis of E. coli fragments, there was no difference between wt and Btk (−/−) BMMs in the uptake of Lm upon infection. Moreover, there was no difference in the response to heat-killed Lm between wt and Btk (−/−) BMMs, suggesting a role for Btk in signaling pathways that are induced by intracellular Lm. Finally, Btk (−/−) mice displayed enhanced resistance and an increased mean survival time upon Lm infection in comparison to wt mice. This correlated with elevated IFN-γ and IL-12p70 serum levels in Btk (−/−) mice at day 1 after infection. Taken together, our data suggest an important regulatory role for Btk in macrophages during Lm infection. Public Library of Science 2013-03-27 /pmc/articles/PMC3609758/ /pubmed/23544144 http://dx.doi.org/10.1371/journal.pone.0060476 Text en © 2013 Köprülü, et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Köprülü, Afitap Derya Kastner, Renate Wienerroither, Sebastian Lassnig, Caroline Putz, Eva Maria Majer, Olivia Reutterer, Benjamin Sexl, Veronika Kuchler, Karl Müller, Mathias Decker, Thomas Ellmeier, Wilfried The Tyrosine Kinase Btk Regulates the Macrophage Response to Listeria monocytogenes Infection |
title | The Tyrosine Kinase Btk Regulates the Macrophage Response to Listeria monocytogenes Infection |
title_full | The Tyrosine Kinase Btk Regulates the Macrophage Response to Listeria monocytogenes Infection |
title_fullStr | The Tyrosine Kinase Btk Regulates the Macrophage Response to Listeria monocytogenes Infection |
title_full_unstemmed | The Tyrosine Kinase Btk Regulates the Macrophage Response to Listeria monocytogenes Infection |
title_short | The Tyrosine Kinase Btk Regulates the Macrophage Response to Listeria monocytogenes Infection |
title_sort | tyrosine kinase btk regulates the macrophage response to listeria monocytogenes infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609758/ https://www.ncbi.nlm.nih.gov/pubmed/23544144 http://dx.doi.org/10.1371/journal.pone.0060476 |
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