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Chito-Oligosaccharide Inhibits the De-Methylation of a ‘CpG’ Island within the Leptin (LEP) Promoter during Adipogenesis of 3T3-L1 Cells

Chito-oligosaccharide (COS) is a natural bioactive compound, which has been shown to suppress lipid metabolic genes and lipid accumulation in differentiating adipocytes. Leptin has been identified as a key regulator of energy homeostasis and is known to be under epigenetic regulation during adipogen...

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Autores principales: Bahar, Bojlul, O’Doherty, John V., O’Doherty, Alan M., Sweeney, Torres
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609775/
https://www.ncbi.nlm.nih.gov/pubmed/23544120
http://dx.doi.org/10.1371/journal.pone.0060011
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author Bahar, Bojlul
O’Doherty, John V.
O’Doherty, Alan M.
Sweeney, Torres
author_facet Bahar, Bojlul
O’Doherty, John V.
O’Doherty, Alan M.
Sweeney, Torres
author_sort Bahar, Bojlul
collection PubMed
description Chito-oligosaccharide (COS) is a natural bioactive compound, which has been shown to suppress lipid metabolic genes and lipid accumulation in differentiating adipocytes. Leptin has been identified as a key regulator of energy homeostasis and is known to be under epigenetic regulation during adipogenesis. Hence, the first objective of this experiment was to compare leptin gene (LEP) expression and leptin secretion during the different stages of adipogenesis and to investigate the effect of COS on these processes. As COS inhibited LEP expression during adipogenesis, the second aim was to investigate the methylation dynamics of a ‘CpG’ island in the proximal region of the LEP promoter during adipogenesis and to determine the effect of COS on this process. Mouse 3T3-L1 cells were stimulated to differentiate in the absence or presence of COS and the levels of leptin mRNA and protein were evaluated on days 0, 2, 4 and 6 post-induction of differentiation (PID). The extent of de-methylation of six CpG sites was evaluated. LEP mRNA transcript and protein could not be detected on either day 0PID or 2PID. In contrast, both were detected on day 4PID (P<0.05) and 6PID (P<0.001) and both were inhibited by COS (P<0.001). Of the six CpG sites analyzed, CpG_52, CpG_62 and CpG_95 became 11.5, 5.0 and 5.0% de-methylated between day 2PID and 6PID, respectively. COS blocked this de-methylation event at CpG_52 (P<0.001), CpG_62 (P<0.01) and CpG_95 (P<0.01) on day 6PID. These data suggest that COS can have an epigenetic effect on differentiating adipocytes, a novel biological function of COS which has potential applications for the manipulation of leptin gene expression, adipogenesis, and conditions within the metabolic syndrome spectrum.
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spelling pubmed-36097752013-03-29 Chito-Oligosaccharide Inhibits the De-Methylation of a ‘CpG’ Island within the Leptin (LEP) Promoter during Adipogenesis of 3T3-L1 Cells Bahar, Bojlul O’Doherty, John V. O’Doherty, Alan M. Sweeney, Torres PLoS One Research Article Chito-oligosaccharide (COS) is a natural bioactive compound, which has been shown to suppress lipid metabolic genes and lipid accumulation in differentiating adipocytes. Leptin has been identified as a key regulator of energy homeostasis and is known to be under epigenetic regulation during adipogenesis. Hence, the first objective of this experiment was to compare leptin gene (LEP) expression and leptin secretion during the different stages of adipogenesis and to investigate the effect of COS on these processes. As COS inhibited LEP expression during adipogenesis, the second aim was to investigate the methylation dynamics of a ‘CpG’ island in the proximal region of the LEP promoter during adipogenesis and to determine the effect of COS on this process. Mouse 3T3-L1 cells were stimulated to differentiate in the absence or presence of COS and the levels of leptin mRNA and protein were evaluated on days 0, 2, 4 and 6 post-induction of differentiation (PID). The extent of de-methylation of six CpG sites was evaluated. LEP mRNA transcript and protein could not be detected on either day 0PID or 2PID. In contrast, both were detected on day 4PID (P<0.05) and 6PID (P<0.001) and both were inhibited by COS (P<0.001). Of the six CpG sites analyzed, CpG_52, CpG_62 and CpG_95 became 11.5, 5.0 and 5.0% de-methylated between day 2PID and 6PID, respectively. COS blocked this de-methylation event at CpG_52 (P<0.001), CpG_62 (P<0.01) and CpG_95 (P<0.01) on day 6PID. These data suggest that COS can have an epigenetic effect on differentiating adipocytes, a novel biological function of COS which has potential applications for the manipulation of leptin gene expression, adipogenesis, and conditions within the metabolic syndrome spectrum. Public Library of Science 2013-03-27 /pmc/articles/PMC3609775/ /pubmed/23544120 http://dx.doi.org/10.1371/journal.pone.0060011 Text en © 2013 Bahar et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bahar, Bojlul
O’Doherty, John V.
O’Doherty, Alan M.
Sweeney, Torres
Chito-Oligosaccharide Inhibits the De-Methylation of a ‘CpG’ Island within the Leptin (LEP) Promoter during Adipogenesis of 3T3-L1 Cells
title Chito-Oligosaccharide Inhibits the De-Methylation of a ‘CpG’ Island within the Leptin (LEP) Promoter during Adipogenesis of 3T3-L1 Cells
title_full Chito-Oligosaccharide Inhibits the De-Methylation of a ‘CpG’ Island within the Leptin (LEP) Promoter during Adipogenesis of 3T3-L1 Cells
title_fullStr Chito-Oligosaccharide Inhibits the De-Methylation of a ‘CpG’ Island within the Leptin (LEP) Promoter during Adipogenesis of 3T3-L1 Cells
title_full_unstemmed Chito-Oligosaccharide Inhibits the De-Methylation of a ‘CpG’ Island within the Leptin (LEP) Promoter during Adipogenesis of 3T3-L1 Cells
title_short Chito-Oligosaccharide Inhibits the De-Methylation of a ‘CpG’ Island within the Leptin (LEP) Promoter during Adipogenesis of 3T3-L1 Cells
title_sort chito-oligosaccharide inhibits the de-methylation of a ‘cpg’ island within the leptin (lep) promoter during adipogenesis of 3t3-l1 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609775/
https://www.ncbi.nlm.nih.gov/pubmed/23544120
http://dx.doi.org/10.1371/journal.pone.0060011
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