Effects of ectopic HER-2/neu gene expression on the COX-2/PGE(2)/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells

OBJECTIVES: To investigate the role of HER-2/neu-mediated COX-2/P450arom signal in estrogen-dependent endometrial carcinoma. METHODS: The recombinant eukaryotic expression vector, pcDNA3.1-HER-2/neu, was constructed and transfect to Ishikawa endometrial carcinoma cells. The expression of COX-2 and P...

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Autores principales: Li, Shu, Ma, XiaoXin, Ma, Li, Wang, Cuicui, He, YuanQi, Yu, ZhiJuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610111/
https://www.ncbi.nlm.nih.gov/pubmed/23453006
http://dx.doi.org/10.1186/1756-9966-32-11
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author Li, Shu
Ma, XiaoXin
Ma, Li
Wang, Cuicui
He, YuanQi
Yu, ZhiJuan
author_facet Li, Shu
Ma, XiaoXin
Ma, Li
Wang, Cuicui
He, YuanQi
Yu, ZhiJuan
author_sort Li, Shu
collection PubMed
description OBJECTIVES: To investigate the role of HER-2/neu-mediated COX-2/P450arom signal in estrogen-dependent endometrial carcinoma. METHODS: The recombinant eukaryotic expression vector, pcDNA3.1-HER-2/neu, was constructed and transfect to Ishikawa endometrial carcinoma cells. The expression of COX-2 and P450arom in transfected cells were detected by real-time PCR and western blotting. The levels of estrogen in cell supernatants were detected by ELISA. RESULTS: Over-expression of HER-2/neu in transfected cells was confirmed by real-time PCR and western blotting. The levels of autocrine estrogen in transfected cells was significantly increased which combination with the enhancement of COX-2 and P450arom expression in transfected cells. CONCLUSION: HER-2/neu induced the improvement of autocrine estrogen in endometrial carcinoma cell through triggering the COX-2/P450arom signal.
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spelling pubmed-36101112013-03-29 Effects of ectopic HER-2/neu gene expression on the COX-2/PGE(2)/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells Li, Shu Ma, XiaoXin Ma, Li Wang, Cuicui He, YuanQi Yu, ZhiJuan J Exp Clin Cancer Res Research OBJECTIVES: To investigate the role of HER-2/neu-mediated COX-2/P450arom signal in estrogen-dependent endometrial carcinoma. METHODS: The recombinant eukaryotic expression vector, pcDNA3.1-HER-2/neu, was constructed and transfect to Ishikawa endometrial carcinoma cells. The expression of COX-2 and P450arom in transfected cells were detected by real-time PCR and western blotting. The levels of estrogen in cell supernatants were detected by ELISA. RESULTS: Over-expression of HER-2/neu in transfected cells was confirmed by real-time PCR and western blotting. The levels of autocrine estrogen in transfected cells was significantly increased which combination with the enhancement of COX-2 and P450arom expression in transfected cells. CONCLUSION: HER-2/neu induced the improvement of autocrine estrogen in endometrial carcinoma cell through triggering the COX-2/P450arom signal. BioMed Central 2013-03-02 /pmc/articles/PMC3610111/ /pubmed/23453006 http://dx.doi.org/10.1186/1756-9966-32-11 Text en Copyright ©2013 Li et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Li, Shu
Ma, XiaoXin
Ma, Li
Wang, Cuicui
He, YuanQi
Yu, ZhiJuan
Effects of ectopic HER-2/neu gene expression on the COX-2/PGE(2)/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells
title Effects of ectopic HER-2/neu gene expression on the COX-2/PGE(2)/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells
title_full Effects of ectopic HER-2/neu gene expression on the COX-2/PGE(2)/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells
title_fullStr Effects of ectopic HER-2/neu gene expression on the COX-2/PGE(2)/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells
title_full_unstemmed Effects of ectopic HER-2/neu gene expression on the COX-2/PGE(2)/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells
title_short Effects of ectopic HER-2/neu gene expression on the COX-2/PGE(2)/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells
title_sort effects of ectopic her-2/neu gene expression on the cox-2/pge(2)/p450arom signaling pathway in endometrial carcinoma cells: her-2/neu gene expression in endometrial carcinoma cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610111/
https://www.ncbi.nlm.nih.gov/pubmed/23453006
http://dx.doi.org/10.1186/1756-9966-32-11
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