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In situ hybridization protocol for enhanced detection of gene expression in the planarian Schmidtea mediterranea

BACKGROUND: The freshwater planarian Schmidtea mediterranea has emerged as a powerful model for studies of regenerative, stem cell, and germ cell biology. Whole-mount in situ hybridization (WISH) and whole-mount fluorescent in situ hybridization (FISH) are critical methods for determining gene expre...

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Detalles Bibliográficos
Autores principales: King, Ryan S, Newmark, Phillip A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610298/
https://www.ncbi.nlm.nih.gov/pubmed/23497040
http://dx.doi.org/10.1186/1471-213X-13-8