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Imaging Beads-Retained Prey Assay for Rapid and Quantitative Protein-Protein Interaction

Conventional Western blot based pull-down methods involve lengthy and laborious work and the results are generally not quantitative. Here, we report the imaging beads-retained prey (IBRP) assay that is rapid and quantitative in studying protein-protein interactions. In this assay, the bait is immobi...

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Detalles Bibliográficos
Autores principales: Zhou, Yan, Hong, Wanjin, Lu, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612083/
https://www.ncbi.nlm.nih.gov/pubmed/23555762
http://dx.doi.org/10.1371/journal.pone.0059727