Robust one-Tube Ω-PCR Strategy Accelerates Precise Sequence Modification of Plasmids for Functional Genomics

Functional genomics requires vector construction for protein expression and functional characterization of target genes; therefore, a simple, flexible and low-cost molecular manipulation strategy will be highly advantageous for genomics approaches. Here, we describe a Ω-PCR strategy that enables mul...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Letian, Wang, Fengpin, Wang, Xiaoyu, Liu, Yao-Guang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Techniques
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612181/
https://www.ncbi.nlm.nih.gov/pubmed/23335613
http://dx.doi.org/10.1093/pcp/pct009

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612181/
https://www.ncbi.nlm.nih.gov/pubmed/23335613
http://dx.doi.org/10.1093/pcp/pct009

Ejemplares similares