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Robust one-Tube Ω-PCR Strategy Accelerates Precise Sequence Modification of Plasmids for Functional Genomics

Functional genomics requires vector construction for protein expression and functional characterization of target genes; therefore, a simple, flexible and low-cost molecular manipulation strategy will be highly advantageous for genomics approaches. Here, we describe a Ω-PCR strategy that enables mul...

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Detalles Bibliográficos
Autores principales:	Chen, Letian, Wang, Fengpin, Wang, Xiaoyu, Liu, Yao-Guang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2013
Materias:	Techniques
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612181/ https://www.ncbi.nlm.nih.gov/pubmed/23335613 http://dx.doi.org/10.1093/pcp/pct009

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