Engineering RNA Endonucleases with Customized Sequence Specificities

Specific cleavage of RNAs is critical for in vitro manipulation of RNA and for in vivo gene silencing. Here we engineer artificial site-specific RNA endonucleases (ASREs) to function analogously to DNA restriction enzymes. We combine a general RNA cleavage domain with a series of Pumilio/FBF (PUF) d...

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Detalles Bibliográficos
Autores principales: Choudhury, Rajarshi, Tsai, Yihsuan S., Dominguez, Daniel, Wang, Yang, Wang, Zefeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612931/
https://www.ncbi.nlm.nih.gov/pubmed/23093184
http://dx.doi.org/10.1038/ncomms2154

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612931/
https://www.ncbi.nlm.nih.gov/pubmed/23093184
http://dx.doi.org/10.1038/ncomms2154

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