Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

BACKGROUND: Inducible high-level expression is favoured for recombinant protein production in Pichia pastoris. Therefore, novel regulated promoters are desired, ideally repressing heterologous gene expression during initial growth and enabling it in the production phase. In a typical large scale fed...

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Detalles Bibliográficos
Autores principales: Prielhofer, Roland, Maurer, Michael, Klein, Joachim, Wenger, Jana, Kiziak, Christoph, Gasser, Brigitte, Mattanovich, Diethard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615954/
https://www.ncbi.nlm.nih.gov/pubmed/23347568
http://dx.doi.org/10.1186/1475-2859-12-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615954/
https://www.ncbi.nlm.nih.gov/pubmed/23347568
http://dx.doi.org/10.1186/1475-2859-12-5

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