Cargando…

Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

BACKGROUND: Inducible high-level expression is favoured for recombinant protein production in Pichia pastoris. Therefore, novel regulated promoters are desired, ideally repressing heterologous gene expression during initial growth and enabling it in the production phase. In a typical large scale fed...

Descripción completa

Detalles Bibliográficos
Autores principales:	Prielhofer, Roland, Maurer, Michael, Klein, Joachim, Wenger, Jana, Kiziak, Christoph, Gasser, Brigitte, Mattanovich, Diethard
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615954/ https://www.ncbi.nlm.nih.gov/pubmed/23347568 http://dx.doi.org/10.1186/1475-2859-12-5

Ejemplares similares

Superior protein titers in half the fermentation time: Promoter and process engineering for the glucose‐regulated GTH1 promoter of Pichia pastoris
por: Prielhofer, Roland, et al.
Publicado: (2018)

Characterization of methanol utilization negative Pichia pastoris for secreted protein production: New cultivation strategies for current and future applications
por: Zavec, Domen, et al.
Publicado: (2020)

Fine-Tuning of Transcription in Pichia pastoris Using dCas9 and RNA Scaffolds
por: Baumschabl, Michael, et al.
Publicado: (2020)

Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level
por: Prielhofer, Roland, et al.
Publicado: (2015)

Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris
por: Moser, Josef W., et al.
Publicado: (2017)

Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris
por: Maurer, Michael, et al.
Publicado: (2006)

Repressible promoters – A novel tool to generate conditional mutants in Pichia pastoris
por: Delic, Marizela, et al.
Publicado: (2013)

Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris
por: Cámara, Elena, et al.
Publicado: (2017)

GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris
por: Prielhofer, Roland, et al.
Publicado: (2017)

The response to unfolded protein is involved in osmotolerance of Pichia pastoris
por: Dragosits, Martin, et al.
Publicado: (2010)

Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions
por: Gasser, Brigitte, et al.
Publicado: (2007)

Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation
por: Sauer, Michael, et al.
Publicado: (2004)

In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response
por: Rebnegger, Corinna, et al.
Publicado: (2014)

Open access to sequence: Browsing the Pichia pastoris genome
por: Mattanovich, Diethard, et al.
Publicado: (2009)

Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris
por: Marx, Hans, et al.
Publicado: (2008)

Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris
por: Marsalek, Lukas, et al.
Publicado: (2019)

Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures
por: Burgard, Jonas, et al.
Publicado: (2017)

Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris
por: Mattanovich, Diethard, et al.
Publicado: (2009)

Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris
por: Nocon, Justyna, et al.
Publicado: (2016)

Functional inclusion bodies produced in the yeast Pichia pastoris
por: Rueda, Fabián, et al.
Publicado: (2016)

Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion
por: Ruth, Claudia, et al.
Publicado: (2014)

Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays
por: Graf, Alexandra, et al.
Publicado: (2008)

Systematic sequence engineering enhances the induction strength of the glucose-regulated GTH1 promoter of Komagataella phaffii
por: Flores-Villegas, Mirelle, et al.
Publicado: (2023)

A multi-level study of recombinant Pichia pastoris in different oxygen conditions
por: Baumann, Kristin, et al.
Publicado: (2010)

The secretome of Pichia pastoris in fed‐batch cultivations is largely independent of the carbon source but changes quantitatively over cultivation time
por: Burgard, Jonas, et al.
Publicado: (2019)

The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO(2)
por: Gassler, Thomas, et al.
Publicado: (2019)

Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins
por: Maccani, Andreas, et al.
Publicado: (2014)

Model based engineering of Pichia pastoris central metabolism enhances recombinant protein production
por: Nocon, Justyna, et al.
Publicado: (2014)

Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel (34)S labeling procedure
por: Pfeffer, Martin, et al.
Publicado: (2011)

A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris
por: Bai, Jiangping, et al.
Publicado: (2011)

Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris
por: Baumann, Kristin, et al.
Publicado: (2011)

Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology
por: Gasser, Brigitte, et al.
Publicado: (2015)

Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels
por: Carnicer, Marc, et al.
Publicado: (2009)

From strain engineering to process development: monoclonal antibody production with an unnatural amino acid in Pichia pastoris
por: Tir, Nora, et al.
Publicado: (2022)

Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris
por: Zepeda, Andrea B., et al.
Publicado: (2014)

Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol
por: Hou, Rui, et al.
Publicado: (2022)

Creation of Stable Heterothallic Strains of Komagataella phaffii Enables Dissection of Mating Gene Regulation
por: Heistinger, Lina, et al.
Publicado: (2017)

Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway
por: Krainer, Florian W, et al.
Publicado: (2012)

Molecular optimization of rabies virus glycoprotein expression in Pichia pastoris
por: Ben Azoun, Safa, et al.
Publicado: (2016)

Pichia pastoris Exhibits High Viability and a Low Maintenance Energy Requirement at Near-Zero Specific Growth Rates
por: Rebnegger, Corinna, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_h1g3qcuumf2g57r00n88mafj8e