MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence
MicroRNAs (miRNAs) are small non-coding RNAs that can posttranscriptionally regulate gene expression by targeting messenger RNAs. During miRNA biogenesis, the star strand (miRNA*) is generally degraded to a low level in the cells. However, certain miRNA* express abundantly and can be recruited into...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616696/ https://www.ncbi.nlm.nih.gov/pubmed/23389951 http://dx.doi.org/10.1093/nar/gkt058 |
_version_ | 1782265153218150400 |
---|---|
author | Tong, Lei Lin, Lexun Wu, Shuo Guo, Zhiwei Wang, Tianying Qin, Ying Wang, Ruixue Zhong, Xiaoyan Wu, Xia Wang, Yan Luan, Tian Wang, Qiang Li, Yunxia Chen, Xiaofeng Zhang, Fengmin Zhao, Wenran Zhong, Zhaohua |
author_facet | Tong, Lei Lin, Lexun Wu, Shuo Guo, Zhiwei Wang, Tianying Qin, Ying Wang, Ruixue Zhong, Xiaoyan Wu, Xia Wang, Yan Luan, Tian Wang, Qiang Li, Yunxia Chen, Xiaofeng Zhang, Fengmin Zhao, Wenran Zhong, Zhaohua |
author_sort | Tong, Lei |
collection | PubMed |
description | MicroRNAs (miRNAs) are small non-coding RNAs that can posttranscriptionally regulate gene expression by targeting messenger RNAs. During miRNA biogenesis, the star strand (miRNA*) is generally degraded to a low level in the cells. However, certain miRNA* express abundantly and can be recruited into the silencing complex to regulate gene expression. Most miRNAs function as suppressive regulators on gene expression. Group B coxsackieviruses (CVB) are the major pathogens of human viral myocarditis and dilated cardiomyopathy. CVB genome is a positive-sense, single-stranded RNA. Our previous study shows that miR-342-5p can suppress CVB biogenesis by targeting its 2C-coding sequence. In this study, we found that the miR-10a duplex could significantly up-regulate the biosynthesis of CVB type 3 (CVB3). Further study showed that it was the miR-10a star strand (miR-10a*) that augmented CVB3 biosynthesis. Site-directed mutagenesis showed that the miR-10a* target was located in the nt6818–nt6941 sequence of the viral 3D-coding region. MiR-10a* was detectable in the cardiac tissues of suckling Balb/c mice, suggesting that miR-10a* may impact CVB3 replication during its cardiac infection. Taken together, these data for the first time show that miRNA* can positively modulate gene expression. MiR-10a* might be involved in the CVB3 cardiac pathogenesis. |
format | Online Article Text |
id | pubmed-3616696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-36166962013-04-04 MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence Tong, Lei Lin, Lexun Wu, Shuo Guo, Zhiwei Wang, Tianying Qin, Ying Wang, Ruixue Zhong, Xiaoyan Wu, Xia Wang, Yan Luan, Tian Wang, Qiang Li, Yunxia Chen, Xiaofeng Zhang, Fengmin Zhao, Wenran Zhong, Zhaohua Nucleic Acids Res RNA MicroRNAs (miRNAs) are small non-coding RNAs that can posttranscriptionally regulate gene expression by targeting messenger RNAs. During miRNA biogenesis, the star strand (miRNA*) is generally degraded to a low level in the cells. However, certain miRNA* express abundantly and can be recruited into the silencing complex to regulate gene expression. Most miRNAs function as suppressive regulators on gene expression. Group B coxsackieviruses (CVB) are the major pathogens of human viral myocarditis and dilated cardiomyopathy. CVB genome is a positive-sense, single-stranded RNA. Our previous study shows that miR-342-5p can suppress CVB biogenesis by targeting its 2C-coding sequence. In this study, we found that the miR-10a duplex could significantly up-regulate the biosynthesis of CVB type 3 (CVB3). Further study showed that it was the miR-10a star strand (miR-10a*) that augmented CVB3 biosynthesis. Site-directed mutagenesis showed that the miR-10a* target was located in the nt6818–nt6941 sequence of the viral 3D-coding region. MiR-10a* was detectable in the cardiac tissues of suckling Balb/c mice, suggesting that miR-10a* may impact CVB3 replication during its cardiac infection. Taken together, these data for the first time show that miRNA* can positively modulate gene expression. MiR-10a* might be involved in the CVB3 cardiac pathogenesis. Oxford University Press 2013-04 2013-02-05 /pmc/articles/PMC3616696/ /pubmed/23389951 http://dx.doi.org/10.1093/nar/gkt058 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | RNA Tong, Lei Lin, Lexun Wu, Shuo Guo, Zhiwei Wang, Tianying Qin, Ying Wang, Ruixue Zhong, Xiaoyan Wu, Xia Wang, Yan Luan, Tian Wang, Qiang Li, Yunxia Chen, Xiaofeng Zhang, Fengmin Zhao, Wenran Zhong, Zhaohua MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence |
title | MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence |
title_full | MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence |
title_fullStr | MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence |
title_full_unstemmed | MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence |
title_short | MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence |
title_sort | mir-10a* up-regulates coxsackievirus b3 biosynthesis by targeting the 3d-coding sequence |
topic | RNA |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616696/ https://www.ncbi.nlm.nih.gov/pubmed/23389951 http://dx.doi.org/10.1093/nar/gkt058 |
work_keys_str_mv | AT tonglei mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT linlexun mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT wushuo mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT guozhiwei mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT wangtianying mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT qinying mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT wangruixue mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT zhongxiaoyan mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT wuxia mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT wangyan mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT luantian mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT wangqiang mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT liyunxia mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT chenxiaofeng mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT zhangfengmin mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT zhaowenran mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence AT zhongzhaohua mir10aupregulatescoxsackievirusb3biosynthesisbytargetingthe3dcodingsequence |