MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence

MicroRNAs (miRNAs) are small non-coding RNAs that can posttranscriptionally regulate gene expression by targeting messenger RNAs. During miRNA biogenesis, the star strand (miRNA*) is generally degraded to a low level in the cells. However, certain miRNA* express abundantly and can be recruited into...

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Detalles Bibliográficos
Autores principales: Tong, Lei, Lin, Lexun, Wu, Shuo, Guo, Zhiwei, Wang, Tianying, Qin, Ying, Wang, Ruixue, Zhong, Xiaoyan, Wu, Xia, Wang, Yan, Luan, Tian, Wang, Qiang, Li, Yunxia, Chen, Xiaofeng, Zhang, Fengmin, Zhao, Wenran, Zhong, Zhaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616696/
https://www.ncbi.nlm.nih.gov/pubmed/23389951
http://dx.doi.org/10.1093/nar/gkt058
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author Tong, Lei
Lin, Lexun
Wu, Shuo
Guo, Zhiwei
Wang, Tianying
Qin, Ying
Wang, Ruixue
Zhong, Xiaoyan
Wu, Xia
Wang, Yan
Luan, Tian
Wang, Qiang
Li, Yunxia
Chen, Xiaofeng
Zhang, Fengmin
Zhao, Wenran
Zhong, Zhaohua
author_facet Tong, Lei
Lin, Lexun
Wu, Shuo
Guo, Zhiwei
Wang, Tianying
Qin, Ying
Wang, Ruixue
Zhong, Xiaoyan
Wu, Xia
Wang, Yan
Luan, Tian
Wang, Qiang
Li, Yunxia
Chen, Xiaofeng
Zhang, Fengmin
Zhao, Wenran
Zhong, Zhaohua
author_sort Tong, Lei
collection PubMed
description MicroRNAs (miRNAs) are small non-coding RNAs that can posttranscriptionally regulate gene expression by targeting messenger RNAs. During miRNA biogenesis, the star strand (miRNA*) is generally degraded to a low level in the cells. However, certain miRNA* express abundantly and can be recruited into the silencing complex to regulate gene expression. Most miRNAs function as suppressive regulators on gene expression. Group B coxsackieviruses (CVB) are the major pathogens of human viral myocarditis and dilated cardiomyopathy. CVB genome is a positive-sense, single-stranded RNA. Our previous study shows that miR-342-5p can suppress CVB biogenesis by targeting its 2C-coding sequence. In this study, we found that the miR-10a duplex could significantly up-regulate the biosynthesis of CVB type 3 (CVB3). Further study showed that it was the miR-10a star strand (miR-10a*) that augmented CVB3 biosynthesis. Site-directed mutagenesis showed that the miR-10a* target was located in the nt6818–nt6941 sequence of the viral 3D-coding region. MiR-10a* was detectable in the cardiac tissues of suckling Balb/c mice, suggesting that miR-10a* may impact CVB3 replication during its cardiac infection. Taken together, these data for the first time show that miRNA* can positively modulate gene expression. MiR-10a* might be involved in the CVB3 cardiac pathogenesis.
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spelling pubmed-36166962013-04-04 MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence Tong, Lei Lin, Lexun Wu, Shuo Guo, Zhiwei Wang, Tianying Qin, Ying Wang, Ruixue Zhong, Xiaoyan Wu, Xia Wang, Yan Luan, Tian Wang, Qiang Li, Yunxia Chen, Xiaofeng Zhang, Fengmin Zhao, Wenran Zhong, Zhaohua Nucleic Acids Res RNA MicroRNAs (miRNAs) are small non-coding RNAs that can posttranscriptionally regulate gene expression by targeting messenger RNAs. During miRNA biogenesis, the star strand (miRNA*) is generally degraded to a low level in the cells. However, certain miRNA* express abundantly and can be recruited into the silencing complex to regulate gene expression. Most miRNAs function as suppressive regulators on gene expression. Group B coxsackieviruses (CVB) are the major pathogens of human viral myocarditis and dilated cardiomyopathy. CVB genome is a positive-sense, single-stranded RNA. Our previous study shows that miR-342-5p can suppress CVB biogenesis by targeting its 2C-coding sequence. In this study, we found that the miR-10a duplex could significantly up-regulate the biosynthesis of CVB type 3 (CVB3). Further study showed that it was the miR-10a star strand (miR-10a*) that augmented CVB3 biosynthesis. Site-directed mutagenesis showed that the miR-10a* target was located in the nt6818–nt6941 sequence of the viral 3D-coding region. MiR-10a* was detectable in the cardiac tissues of suckling Balb/c mice, suggesting that miR-10a* may impact CVB3 replication during its cardiac infection. Taken together, these data for the first time show that miRNA* can positively modulate gene expression. MiR-10a* might be involved in the CVB3 cardiac pathogenesis. Oxford University Press 2013-04 2013-02-05 /pmc/articles/PMC3616696/ /pubmed/23389951 http://dx.doi.org/10.1093/nar/gkt058 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Tong, Lei
Lin, Lexun
Wu, Shuo
Guo, Zhiwei
Wang, Tianying
Qin, Ying
Wang, Ruixue
Zhong, Xiaoyan
Wu, Xia
Wang, Yan
Luan, Tian
Wang, Qiang
Li, Yunxia
Chen, Xiaofeng
Zhang, Fengmin
Zhao, Wenran
Zhong, Zhaohua
MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence
title MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence
title_full MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence
title_fullStr MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence
title_full_unstemmed MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence
title_short MiR-10a* up-regulates coxsackievirus B3 biosynthesis by targeting the 3D-coding sequence
title_sort mir-10a* up-regulates coxsackievirus b3 biosynthesis by targeting the 3d-coding sequence
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616696/
https://www.ncbi.nlm.nih.gov/pubmed/23389951
http://dx.doi.org/10.1093/nar/gkt058
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