Cargando…
Molecular Mechanisms of HMW Glutenin Subunits from 1S(l) Genome of Aegilops longissima Positively Affecting Wheat Breadmaking Quality
A wheat cultivar “Chinese Spring” chromosome substitution line CS-1S(l)(1B), in which the 1B chromosome was substituted by 1S(l) from Aegilops longissima, was developed and found to possess superior dough and breadmaking quality. The molecular mechanism of its super quality conformation is studied i...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617193/ https://www.ncbi.nlm.nih.gov/pubmed/23593125 http://dx.doi.org/10.1371/journal.pone.0058947 |
_version_ | 1782265235755761664 |
---|---|
author | Wang, Shunli Yu, Zitong Cao, Min Shen, Xixi Li, Ning Li, Xiaohui Ma, Wujun Weißgerber, H. Zeller, Friedrich Hsam, Sai Yan, Yueming |
author_facet | Wang, Shunli Yu, Zitong Cao, Min Shen, Xixi Li, Ning Li, Xiaohui Ma, Wujun Weißgerber, H. Zeller, Friedrich Hsam, Sai Yan, Yueming |
author_sort | Wang, Shunli |
collection | PubMed |
description | A wheat cultivar “Chinese Spring” chromosome substitution line CS-1S(l)(1B), in which the 1B chromosome was substituted by 1S(l) from Aegilops longissima, was developed and found to possess superior dough and breadmaking quality. The molecular mechanism of its super quality conformation is studied in the aspects of high molecular glutenin genes, protein accumulation patterns, glutenin polymeric proteins, protein bodies, starch granules, and protein disulfide isomerase (PDI) and PDI-like protein expressions. Results showed that the introduced HMW-GS 1S(l)×2.3* and 1S(l)y16* in the substitution line possesses long repetitive domain, making both be larger than any known x- and y-type subunits from B genome. The introduced subunit genes were also found to have a higher level of mRNA expressions during grain development, resulting in more HMW-GS accumulation in the mature grains. A higher abundance of PDI and PDI-like proteins was observed which possess a known function of assisting disulfide bond formation. Larger HMW-GS deposited in protein bodies were also found in the substitution line. The CS substitution line is expected to be highly valuable in wheat quality improvement since the novel HMW-GS are located on chromosome 1S(l), making it possible to combine with the known superior D×5+Dy10 subunits encoded by Glu-D1 for developing high quality bread wheat. |
format | Online Article Text |
id | pubmed-3617193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36171932013-04-16 Molecular Mechanisms of HMW Glutenin Subunits from 1S(l) Genome of Aegilops longissima Positively Affecting Wheat Breadmaking Quality Wang, Shunli Yu, Zitong Cao, Min Shen, Xixi Li, Ning Li, Xiaohui Ma, Wujun Weißgerber, H. Zeller, Friedrich Hsam, Sai Yan, Yueming PLoS One Research Article A wheat cultivar “Chinese Spring” chromosome substitution line CS-1S(l)(1B), in which the 1B chromosome was substituted by 1S(l) from Aegilops longissima, was developed and found to possess superior dough and breadmaking quality. The molecular mechanism of its super quality conformation is studied in the aspects of high molecular glutenin genes, protein accumulation patterns, glutenin polymeric proteins, protein bodies, starch granules, and protein disulfide isomerase (PDI) and PDI-like protein expressions. Results showed that the introduced HMW-GS 1S(l)×2.3* and 1S(l)y16* in the substitution line possesses long repetitive domain, making both be larger than any known x- and y-type subunits from B genome. The introduced subunit genes were also found to have a higher level of mRNA expressions during grain development, resulting in more HMW-GS accumulation in the mature grains. A higher abundance of PDI and PDI-like proteins was observed which possess a known function of assisting disulfide bond formation. Larger HMW-GS deposited in protein bodies were also found in the substitution line. The CS substitution line is expected to be highly valuable in wheat quality improvement since the novel HMW-GS are located on chromosome 1S(l), making it possible to combine with the known superior D×5+Dy10 subunits encoded by Glu-D1 for developing high quality bread wheat. Public Library of Science 2013-04-04 /pmc/articles/PMC3617193/ /pubmed/23593125 http://dx.doi.org/10.1371/journal.pone.0058947 Text en © 2013 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wang, Shunli Yu, Zitong Cao, Min Shen, Xixi Li, Ning Li, Xiaohui Ma, Wujun Weißgerber, H. Zeller, Friedrich Hsam, Sai Yan, Yueming Molecular Mechanisms of HMW Glutenin Subunits from 1S(l) Genome of Aegilops longissima Positively Affecting Wheat Breadmaking Quality |
title | Molecular Mechanisms of HMW Glutenin Subunits from 1S(l) Genome of Aegilops longissima Positively Affecting Wheat Breadmaking Quality |
title_full | Molecular Mechanisms of HMW Glutenin Subunits from 1S(l) Genome of Aegilops longissima Positively Affecting Wheat Breadmaking Quality |
title_fullStr | Molecular Mechanisms of HMW Glutenin Subunits from 1S(l) Genome of Aegilops longissima Positively Affecting Wheat Breadmaking Quality |
title_full_unstemmed | Molecular Mechanisms of HMW Glutenin Subunits from 1S(l) Genome of Aegilops longissima Positively Affecting Wheat Breadmaking Quality |
title_short | Molecular Mechanisms of HMW Glutenin Subunits from 1S(l) Genome of Aegilops longissima Positively Affecting Wheat Breadmaking Quality |
title_sort | molecular mechanisms of hmw glutenin subunits from 1s(l) genome of aegilops longissima positively affecting wheat breadmaking quality |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617193/ https://www.ncbi.nlm.nih.gov/pubmed/23593125 http://dx.doi.org/10.1371/journal.pone.0058947 |
work_keys_str_mv | AT wangshunli molecularmechanismsofhmwgluteninsubunitsfrom1slgenomeofaegilopslongissimapositivelyaffectingwheatbreadmakingquality AT yuzitong molecularmechanismsofhmwgluteninsubunitsfrom1slgenomeofaegilopslongissimapositivelyaffectingwheatbreadmakingquality AT caomin molecularmechanismsofhmwgluteninsubunitsfrom1slgenomeofaegilopslongissimapositivelyaffectingwheatbreadmakingquality AT shenxixi molecularmechanismsofhmwgluteninsubunitsfrom1slgenomeofaegilopslongissimapositivelyaffectingwheatbreadmakingquality AT lining molecularmechanismsofhmwgluteninsubunitsfrom1slgenomeofaegilopslongissimapositivelyaffectingwheatbreadmakingquality AT lixiaohui molecularmechanismsofhmwgluteninsubunitsfrom1slgenomeofaegilopslongissimapositivelyaffectingwheatbreadmakingquality AT mawujun molecularmechanismsofhmwgluteninsubunitsfrom1slgenomeofaegilopslongissimapositivelyaffectingwheatbreadmakingquality AT weißgerberh molecularmechanismsofhmwgluteninsubunitsfrom1slgenomeofaegilopslongissimapositivelyaffectingwheatbreadmakingquality AT zellerfriedrich molecularmechanismsofhmwgluteninsubunitsfrom1slgenomeofaegilopslongissimapositivelyaffectingwheatbreadmakingquality AT hsamsai molecularmechanismsofhmwgluteninsubunitsfrom1slgenomeofaegilopslongissimapositivelyaffectingwheatbreadmakingquality AT yanyueming molecularmechanismsofhmwgluteninsubunitsfrom1slgenomeofaegilopslongissimapositivelyaffectingwheatbreadmakingquality |