Bacterial expression strategies for several Sus scrofa diacylglycerol kinase alpha constructs: solubility challenges

We pursued several strategies for expressing either full-length Sus scrofa diacylglycerol kinase (DGK) alpha or the catalytic domain (alphacat) in Escherichia coli. Alphacat could be extracted, refolded, and purified from inclusion bodies, but when subjected to analytical gel filtration chromatograp...

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Detalles Bibliográficos
Autores principales: Petro, Elizabeth J., Raben, Daniel M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617429/
https://www.ncbi.nlm.nih.gov/pubmed/23558375
http://dx.doi.org/10.1038/srep01609

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617429/
https://www.ncbi.nlm.nih.gov/pubmed/23558375
http://dx.doi.org/10.1038/srep01609

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