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Bacterial expression strategies for several Sus scrofa diacylglycerol kinase alpha constructs: solubility challenges
We pursued several strategies for expressing either full-length Sus scrofa diacylglycerol kinase (DGK) alpha or the catalytic domain (alphacat) in Escherichia coli. Alphacat could be extracted, refolded, and purified from inclusion bodies, but when subjected to analytical gel filtration chromatograp...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617429/ https://www.ncbi.nlm.nih.gov/pubmed/23558375 http://dx.doi.org/10.1038/srep01609 |