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High-throughput fluorescent-based NKCC functional assay in adherent epithelial cells

BACKGROUND: The kidney-specific NKCC cotransporter isoform NKCC2 is involved in the Na(+) reabsorption in the Thich Ascending Limb (TAL) cells and in the regulation of body fluid volume. In contrast, the isoform NKCC1 represents the major pathway for Cl(-) entry in endothelial cells, playing a cruci...

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Detalles Bibliográficos
Autores principales: Carmosino, Monica, Rizzo, Federica, Torretta, Silvia, Procino, Giuseppe, Svelto, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3618206/
https://www.ncbi.nlm.nih.gov/pubmed/23506056
http://dx.doi.org/10.1186/1471-2121-14-16