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Analysis of Sec61p and Ssh1p interactions in the ER membrane using the split-ubiquitin system
BACKGROUND: The split-ubiquitin system monitors interactions of transmembrane proteins in yeast. It is based on the formation of a quasi-native ubiquitin structure upon interaction of two proteins to which the N- and C-terminal halves of ubiquitin have been fused. In the system we use here ubiquitin...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3618304/ https://www.ncbi.nlm.nih.gov/pubmed/23497013 http://dx.doi.org/10.1186/1471-2121-14-14 |