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TRIM16 overexpression induces apoptosis through activation of caspase-2 in cancer cells

TRIM16 exhibits tumour suppressor functions by interacting with cytoplasmic vimentin and nuclear E2F1 proteins in neuroblastoma and squamous cell carcinoma cells, reducing cell migration and replication. Reduced TRIM16 expression in a range of human primary malignant tissues correlates with increase...

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Autores principales: Kim, Patrick Y., Rahmanto, Aldwin Suryo, Tan, Owen, Norris, Murray D., Haber, Michelle, Marshall, Glenn M., Cheung, Belamy B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3618413/
https://www.ncbi.nlm.nih.gov/pubmed/23404198
http://dx.doi.org/10.1007/s10495-013-0813-y
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author Kim, Patrick Y.
Rahmanto, Aldwin Suryo
Tan, Owen
Norris, Murray D.
Haber, Michelle
Marshall, Glenn M.
Cheung, Belamy B.
author_facet Kim, Patrick Y.
Rahmanto, Aldwin Suryo
Tan, Owen
Norris, Murray D.
Haber, Michelle
Marshall, Glenn M.
Cheung, Belamy B.
author_sort Kim, Patrick Y.
collection PubMed
description TRIM16 exhibits tumour suppressor functions by interacting with cytoplasmic vimentin and nuclear E2F1 proteins in neuroblastoma and squamous cell carcinoma cells, reducing cell migration and replication. Reduced TRIM16 expression in a range of human primary malignant tissues correlates with increased malignant potential. TRIM16 also induces apoptosis in breast and lung cancer cells, by unknown mechanisms. Here we show that overexpression of TRIM16 induces apoptosis in human breast cancer (MCF7) and neuroblastoma (BE(2)-C) cells, but not in non-malignant HEK293 cells. TRIM16 increased procaspase-2 protein levels in MCF7 and induced caspase-2 activity in both MCF7 and BE(2)-C cells. We show that TRIM16 and caspase-2 proteins directly interact in both MCF7 and BE(2)-C cells and co-localise in MCF7 cells. Most importantly, the induction of caspase-2 activity is required for TRIM16 to initiate apoptosis. Our data suggest a novel mechanism by which TRIM16 can promote apoptosis by directly modulating caspase-2 activity.
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spelling pubmed-36184132013-04-08 TRIM16 overexpression induces apoptosis through activation of caspase-2 in cancer cells Kim, Patrick Y. Rahmanto, Aldwin Suryo Tan, Owen Norris, Murray D. Haber, Michelle Marshall, Glenn M. Cheung, Belamy B. Apoptosis Original Paper TRIM16 exhibits tumour suppressor functions by interacting with cytoplasmic vimentin and nuclear E2F1 proteins in neuroblastoma and squamous cell carcinoma cells, reducing cell migration and replication. Reduced TRIM16 expression in a range of human primary malignant tissues correlates with increased malignant potential. TRIM16 also induces apoptosis in breast and lung cancer cells, by unknown mechanisms. Here we show that overexpression of TRIM16 induces apoptosis in human breast cancer (MCF7) and neuroblastoma (BE(2)-C) cells, but not in non-malignant HEK293 cells. TRIM16 increased procaspase-2 protein levels in MCF7 and induced caspase-2 activity in both MCF7 and BE(2)-C cells. We show that TRIM16 and caspase-2 proteins directly interact in both MCF7 and BE(2)-C cells and co-localise in MCF7 cells. Most importantly, the induction of caspase-2 activity is required for TRIM16 to initiate apoptosis. Our data suggest a novel mechanism by which TRIM16 can promote apoptosis by directly modulating caspase-2 activity. Springer US 2013-02-13 2013 /pmc/articles/PMC3618413/ /pubmed/23404198 http://dx.doi.org/10.1007/s10495-013-0813-y Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Paper
Kim, Patrick Y.
Rahmanto, Aldwin Suryo
Tan, Owen
Norris, Murray D.
Haber, Michelle
Marshall, Glenn M.
Cheung, Belamy B.
TRIM16 overexpression induces apoptosis through activation of caspase-2 in cancer cells
title TRIM16 overexpression induces apoptosis through activation of caspase-2 in cancer cells
title_full TRIM16 overexpression induces apoptosis through activation of caspase-2 in cancer cells
title_fullStr TRIM16 overexpression induces apoptosis through activation of caspase-2 in cancer cells
title_full_unstemmed TRIM16 overexpression induces apoptosis through activation of caspase-2 in cancer cells
title_short TRIM16 overexpression induces apoptosis through activation of caspase-2 in cancer cells
title_sort trim16 overexpression induces apoptosis through activation of caspase-2 in cancer cells
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3618413/
https://www.ncbi.nlm.nih.gov/pubmed/23404198
http://dx.doi.org/10.1007/s10495-013-0813-y
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