A Duplex PCR-Based Assay for Measuring the Amount of Bacterial Contamination in a Nucleic Acid Extract from a Culture of Free-Living Protists

BACKGROUND: Cultures of heterotrophic protists often require co-culturing with bacteria to act as a source of nutrition. Such cultures will contain varying levels of intrinsic bacterial contamination that can interfere with molecular research and cause problems with the collection of sufficient mate...

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Detalles Bibliográficos
Autores principales: Marron, Alan O., Akam, Michael, Walker, Giselle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3625144/
https://www.ncbi.nlm.nih.gov/pubmed/23593495
http://dx.doi.org/10.1371/journal.pone.0061732

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3625144/
https://www.ncbi.nlm.nih.gov/pubmed/23593495
http://dx.doi.org/10.1371/journal.pone.0061732

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