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Activated charcoal-mediated RNA extraction method for Azadirachta indica and plants highly rich in polyphenolics, polysaccharides and other complex secondary compounds

BACKGROUND: High quality RNA is a primary requisite for numerous molecular biological applications but is difficult to isolate from several plants rich in polysaccharides, polyphenolics and other secondary metabolites. These compounds either bind with nucleic acids or often co-precipitate at the fin...

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Detalles Bibliográficos
Autores principales: Rajakani, Raja, Narnoliya, Lokesh, Sangwan, Neelam Singh, Sangwan, Rajender Singh, Gupta, Vikrant
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626780/
https://www.ncbi.nlm.nih.gov/pubmed/23537338
http://dx.doi.org/10.1186/1756-0500-6-125