Cargando…

Does the essential oil of Lippia sidoides Cham. (pepper-rosmarin) affect its endophytic microbial community?

BACKGROUND: Lippia sidoides Cham., also known as pepper-rosmarin, produces an essential oil in its leaves that is currently used by the pharmaceutical, perfumery and cosmetic industries for its antimicrobial and aromatic properties. Because of the antimicrobial compounds (mainly thymol and carvacrol...

Descripción completa

Detalles Bibliográficos
Autores principales: da Silva, Thais Freitas, Vollú, Renata Estebanez, Jurelevicius, Diogo, Alviano, Daniela Sales, Alviano, Celuta Sales, Blank, Arie Fitzgerald, Seldin, Lucy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626855/
https://www.ncbi.nlm.nih.gov/pubmed/23387945
http://dx.doi.org/10.1186/1471-2180-13-29
_version_ 1782266256956588032
author da Silva, Thais Freitas
Vollú, Renata Estebanez
Jurelevicius, Diogo
Alviano, Daniela Sales
Alviano, Celuta Sales
Blank, Arie Fitzgerald
Seldin, Lucy
author_facet da Silva, Thais Freitas
Vollú, Renata Estebanez
Jurelevicius, Diogo
Alviano, Daniela Sales
Alviano, Celuta Sales
Blank, Arie Fitzgerald
Seldin, Lucy
author_sort da Silva, Thais Freitas
collection PubMed
description BACKGROUND: Lippia sidoides Cham., also known as pepper-rosmarin, produces an essential oil in its leaves that is currently used by the pharmaceutical, perfumery and cosmetic industries for its antimicrobial and aromatic properties. Because of the antimicrobial compounds (mainly thymol and carvacrol) found in the essential oil, we believe that the endophytic microorganisms found in L. sidoides are selected to live in different parts of the plant. RESULTS: In this study, the endophytic microbial communities from the stems and leaves of four L. sidoides genotypes were determined using cultivation-dependent and cultivation-independent approaches. In total, 145 endophytic bacterial strains were isolated and further grouped using either ERIC-PCR or BOX-PCR, resulting in 76 groups composed of different genera predominantly belonging to the Gammaproteobacteria. The endophytic microbial diversity was also analyzed by PCR-DGGE using 16S rRNA-based universal and group-specific primers for total bacteria, Alphaproteobacteria, Betaproteobacteria and Actinobacteria and 18S rRNA-based primers for fungi. PCR-DGGE profile analysis and principal component analysis showed that the total bacteria, Alphaproteobacteria, Betaproteobacteria and fungi were influenced not only by the location within the plant (leaf vs. stem) but also by the presence of the main components of the L. sidoides essential oil (thymol and/or carvacrol) in the leaves. However, the same could not be observed within the Actinobacteria. CONCLUSION: The data presented here are the first step to begin shedding light on the impact of the essential oil in the endophytic microorganisms in pepper-rosmarin.
format Online
Article
Text
id pubmed-3626855
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-36268552013-04-16 Does the essential oil of Lippia sidoides Cham. (pepper-rosmarin) affect its endophytic microbial community? da Silva, Thais Freitas Vollú, Renata Estebanez Jurelevicius, Diogo Alviano, Daniela Sales Alviano, Celuta Sales Blank, Arie Fitzgerald Seldin, Lucy BMC Microbiol Research Article BACKGROUND: Lippia sidoides Cham., also known as pepper-rosmarin, produces an essential oil in its leaves that is currently used by the pharmaceutical, perfumery and cosmetic industries for its antimicrobial and aromatic properties. Because of the antimicrobial compounds (mainly thymol and carvacrol) found in the essential oil, we believe that the endophytic microorganisms found in L. sidoides are selected to live in different parts of the plant. RESULTS: In this study, the endophytic microbial communities from the stems and leaves of four L. sidoides genotypes were determined using cultivation-dependent and cultivation-independent approaches. In total, 145 endophytic bacterial strains were isolated and further grouped using either ERIC-PCR or BOX-PCR, resulting in 76 groups composed of different genera predominantly belonging to the Gammaproteobacteria. The endophytic microbial diversity was also analyzed by PCR-DGGE using 16S rRNA-based universal and group-specific primers for total bacteria, Alphaproteobacteria, Betaproteobacteria and Actinobacteria and 18S rRNA-based primers for fungi. PCR-DGGE profile analysis and principal component analysis showed that the total bacteria, Alphaproteobacteria, Betaproteobacteria and fungi were influenced not only by the location within the plant (leaf vs. stem) but also by the presence of the main components of the L. sidoides essential oil (thymol and/or carvacrol) in the leaves. However, the same could not be observed within the Actinobacteria. CONCLUSION: The data presented here are the first step to begin shedding light on the impact of the essential oil in the endophytic microorganisms in pepper-rosmarin. BioMed Central 2013-02-07 /pmc/articles/PMC3626855/ /pubmed/23387945 http://dx.doi.org/10.1186/1471-2180-13-29 Text en Copyright © 2013 da Silva et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
da Silva, Thais Freitas
Vollú, Renata Estebanez
Jurelevicius, Diogo
Alviano, Daniela Sales
Alviano, Celuta Sales
Blank, Arie Fitzgerald
Seldin, Lucy
Does the essential oil of Lippia sidoides Cham. (pepper-rosmarin) affect its endophytic microbial community?
title Does the essential oil of Lippia sidoides Cham. (pepper-rosmarin) affect its endophytic microbial community?
title_full Does the essential oil of Lippia sidoides Cham. (pepper-rosmarin) affect its endophytic microbial community?
title_fullStr Does the essential oil of Lippia sidoides Cham. (pepper-rosmarin) affect its endophytic microbial community?
title_full_unstemmed Does the essential oil of Lippia sidoides Cham. (pepper-rosmarin) affect its endophytic microbial community?
title_short Does the essential oil of Lippia sidoides Cham. (pepper-rosmarin) affect its endophytic microbial community?
title_sort does the essential oil of lippia sidoides cham. (pepper-rosmarin) affect its endophytic microbial community?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626855/
https://www.ncbi.nlm.nih.gov/pubmed/23387945
http://dx.doi.org/10.1186/1471-2180-13-29
work_keys_str_mv AT dasilvathaisfreitas doestheessentialoiloflippiasidoideschampepperrosmarinaffectitsendophyticmicrobialcommunity
AT vollurenataestebanez doestheessentialoiloflippiasidoideschampepperrosmarinaffectitsendophyticmicrobialcommunity
AT jureleviciusdiogo doestheessentialoiloflippiasidoideschampepperrosmarinaffectitsendophyticmicrobialcommunity
AT alvianodanielasales doestheessentialoiloflippiasidoideschampepperrosmarinaffectitsendophyticmicrobialcommunity
AT alvianocelutasales doestheessentialoiloflippiasidoideschampepperrosmarinaffectitsendophyticmicrobialcommunity
AT blankariefitzgerald doestheessentialoiloflippiasidoideschampepperrosmarinaffectitsendophyticmicrobialcommunity
AT seldinlucy doestheessentialoiloflippiasidoideschampepperrosmarinaffectitsendophyticmicrobialcommunity