Inhibition of BCR/ABL Protein Expression by miR-203 Sensitizes for Imatinib Mesylate

Selective inhibition of BCR/ABL expression by RNA interference has been demonstrated as an effective strategy in CML treatment and a reversal to imatinib resistance. microRNAs (miRNAs) are small regulatory RNAs involved in post-transcriptional gene regulation. miR-203 is supposed to directly regulat...

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Detalles Bibliográficos
Autores principales: Li, Yajuan, Yuan, Ying, Tao, Kun, Wang, Xin, Xiao, Qing, Huang, Zhenglan, Zhong, Liang, Cao, Weixi, Wen, Jianping, Feng, Wenli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3627914/
https://www.ncbi.nlm.nih.gov/pubmed/23613955
http://dx.doi.org/10.1371/journal.pone.0061858
Descripción
Sumario:Selective inhibition of BCR/ABL expression by RNA interference has been demonstrated as an effective strategy in CML treatment and a reversal to imatinib resistance. microRNAs (miRNAs) are small regulatory RNAs involved in post-transcriptional gene regulation. miR-203 is supposed to directly regulate ABL and BCR/ABL expression, however, the role of miR-203 in imatinib-resistant cells is not clear. Here, we report that overexpression of miR-203 in BaF3-BCR/ABL cells with T315I mutant inhibited cell growth and colony formation ability. Furthermore, miR-203 increased sensitivity to imatinib in BaF3-BCR/ABL(T315I) cells, thereby antagonizing the main mechanism of resistance to imatinib.

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