Cargando…

Enhanced De Novo Assembly of High Throughput Pyrosequencing Data Using Whole Genome Mapping

Despite major advances in next-generation sequencing, assembly of sequencing data, especially data from novel microorganisms or re-emerging pathogens, remains constrained by the lack of suitable reference sequences. De novo assembly is the best approach to achieve an accurate finished sequence, but...

Descripción completa

Detalles Bibliográficos
Autores principales:	Onmus-Leone, Fatma, Hang, Jun, Clifford, Robert J., Yang, Yu, Riley, Matthew C., Kuschner, Robert A., Waterman, Paige E., Lesho, Emil P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3629165/ https://www.ncbi.nlm.nih.gov/pubmed/23613926 http://dx.doi.org/10.1371/journal.pone.0061762

Ejemplares similares

1670 Comparative Metagenomics of Polymicrobial Surface Organisms and Their Invasive Monomicrobial Analogs Indicates the Rate of Genetic Alterations in the Infectious Organisms not Influenced by the Local Microbiome
por: Nielsen, Lindsey, et al.
Publicado: (2014)

1671 A Fatal Outbreak of a Rare but Emerging Clone of Extensively Drug-resistant Acinetobacter baumannii with Enhanced Virulence
por: Jones, Crystal, et al.
Publicado: (2014)

Amplification of Aminoglycoside Resistance Gene aphA1 in Acinetobacter baumannii Results in Tobramycin Therapy Failure
por: McGann, Patrick, et al.
Publicado: (2014)

Whole-Genome Analysis of Bartonella ancashensis, a Novel Pathogen Causing Verruga Peruana, Rural Ancash Region, Peru
por: Mullins, Kristin E., et al.
Publicado: (2017)

Genome Sequencing of Pathogenic Rhodococcus spp.
por: Hang, Jun, et al.
Publicado: (2012)

The Challenges of Implementing Next Generation Sequencing Across a Large Healthcare System, and the Molecular Epidemiology and Antibiotic Susceptibilities of Carbapenemase-Producing Bacteria in the Healthcare System of the U.S. Department of Defense
por: Lesho, Emil, et al.
Publicado: (2016)

Rapid whole genome optical mapping of Plasmodium falciparum
por: Riley, Matthew C, et al.
Publicado: (2011)

Complete Genome Sequence of Bartonella ancashensis Strain 20.00, Isolated from the Blood of a Patient with Verruga Peruana
por: Hang, Jun, et al.
Publicado: (2015)

Anatomic, Geographic, and Taxon-Specific Relative Risks of Carbapenem Resistance in the Health Care System of the U.S. Department of Defense
por: Lesho, Emil, et al.
Publicado: (2016)

A garter snake transcriptome: pyrosequencing, de novo assembly, and sex-specific differences
por: Schwartz, Tonia S, et al.
Publicado: (2010)

Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run
por: Riley, Matthew C., et al.
Publicado: (2011)

Data handling strategies for high throughput pyrosequencers
por: Trombetti, Gabriele A, et al.
Publicado: (2007)

De novo Assembly and Characterization of the Barnyardgrass (Echinochloa crus-galli) Transcriptome Using Next-Generation Pyrosequencing
por: Yang, Xia, et al.
Publicado: (2013)

Employing whole genome mapping for optimal de novo assembly of bacterial genomes
por: Xavier, Basil Britto, et al.
Publicado: (2014)

Aggressive assembly of pyrosequencing reads with mates
por: Miller, Jason R., et al.
Publicado: (2008)

High Throughput Automated Allele Frequency Estimation by Pyrosequencing
por: Doostzadeh, Julie, et al.
Publicado: (2008)

Detection of Bacterial 16S rRNA and Identification of Four Clinically Important Bacteria by Real-Time PCR
por: Clifford, Robert J., et al.
Publicado: (2012)

16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles
por: Hang, Jun, et al.
Publicado: (2014)

De novo Sequence Assembly and Characterization of Lycoris aurea Transcriptome Using GS FLX Titanium Platform of 454 Pyrosequencing
por: Wang, Ren, et al.
Publicado: (2013)

SNP discovery in swine by reduced representation and high throughput pyrosequencing
por: Wiedmann, Ralph T, et al.
Publicado: (2008)

Arbovirus Detection in Insect Vectors by Rapid, High-Throughput Pyrosequencing
por: Bishop-Lilly, Kimberly A., et al.
Publicado: (2010)

Empirical assessment of sequencing errors for high throughput pyrosequencing data
por: da Fonseca, Paulo GS, et al.
Publicado: (2013)

A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers
por: Schafleitner, Roland, et al.
Publicado: (2010)

Pyrosequencing and de novo assembly of Antarctic krill (Euphausia superba) transcriptome to study the adaptability of krill to climate-induced environmental changes
por: Meyer, B, et al.
Publicado: (2015)

Analysis of the melon (Cucumis melo) small RNAome by high-throughput pyrosequencing
por: Gonzalez-Ibeas, Daniel, et al.
Publicado: (2011)

A simple method using Pyrosequencing(TM) to identify de novo SNPs in pooled DNA samples
por: Lin, Yeong-Shin, et al.
Publicado: (2011)

High-Throughput Sequencing and De Novo Assembly of the Isatis indigotica Transcriptome
por: Tang, Xiaoqing, et al.
Publicado: (2014)

In Vivo Fitness Adaptations of Colistin-Resistant Acinetobacter baumannii Isolates to Oxidative Stress
por: Jones, Crystal L., et al.
Publicado: (2017)

Whole Genome Amplification and De novo Assembly of Single Bacterial Cells
por: Rodrigue, Sébastien, et al.
Publicado: (2009)

Deep sampling of the Palomero maize transcriptome by a high throughput strategy of pyrosequencing
por: Vega-Arreguín, Julio C, et al.
Publicado: (2009)

Comparative analysis of bacterial community structure in the rhizosphere of maize by high-throughput pyrosequencing
por: Yang, Yi, et al.
Publicado: (2017)

De novo transcriptome analysis using 454 pyrosequencing of the Himalayan Mayapple, Podophyllum hexandrum
por: Bhattacharyya, Dipto, et al.
Publicado: (2013)

De novo assembly and transcriptome analysis of five major tissues of Jatropha curcas L. using GS FLX titanium platform of 454 pyrosequencing
por: Natarajan, Purushothaman, et al.
Publicado: (2011)

83. During A Million Patient-Days of Surveillance, Low Levels of Infection Prevention Staff Correlated with Higher Rates of Some Healthcare-Associated Infections
por: Lesho, Emil P, et al.
Publicado: (2019)

Methicillin-Resistant Staphylococcus aureus as a Probable Cause of Antibiotic-Associated Enterocolitis
por: Lane, Alison B., et al.
Publicado: (2018)

Hybrid de novo whole-genome assembly and annotation of the model tapeworm Hymenolepis diminuta
por: Nowak, Robert M., et al.
Publicado: (2019)

Methylation enrichment pyrosequencing: combining the specificity of MSP with validation by pyrosequencing
por: Shaw, Richard J., et al.
Publicado: (2006)

De Novo Assembly and Transcriptome Characterization of Canine Retina Using High-Throughput Sequencing
por: Reddy, Bhaskar, et al.
Publicado: (2015)

Robust high-throughput prokaryote de novo assembly and improvement pipeline for Illumina data
por: Page, Andrew J., et al.
Publicado: (2016)

De Novo Assembly and Characterization of the Fruit Transcriptome of Chinese Jujube (Ziziphus jujuba Mill.) Using 454 Pyrosequencing and the Development of Novel Tri-Nucleotide SSR Markers
por: Li, Yingyue, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_t5o8blhu42siaob2mptbm2878g