Steroid sulphatase and oestrogen sulphotransferase in human non-small-cell lung carcinoma

BACKGROUND: Steroid sulphatase (STS) is one of the steroid-metabolising enzymes involved in desulphating inactive steroid sulphates and oestrogen sulphotransferase (EST) sulphates active oestrogen. The roles of both STS and EST have not been examined in oestrogen-dependent non-small-cell lung cancer...

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Autores principales: Iida, S, Kakinuma, H, Miki, Y, Abe, K, Sakurai, M, Suzuki, S, Niikawa, H, Akahira, J, Suzuki, T, Sasano, H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3629433/
https://www.ncbi.nlm.nih.gov/pubmed/23531699
http://dx.doi.org/10.1038/bjc.2013.84
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author Iida, S
Kakinuma, H
Miki, Y
Abe, K
Sakurai, M
Suzuki, S
Niikawa, H
Akahira, J
Suzuki, T
Sasano, H
author_facet Iida, S
Kakinuma, H
Miki, Y
Abe, K
Sakurai, M
Suzuki, S
Niikawa, H
Akahira, J
Suzuki, T
Sasano, H
author_sort Iida, S
collection PubMed
description BACKGROUND: Steroid sulphatase (STS) is one of the steroid-metabolising enzymes involved in desulphating inactive steroid sulphates and oestrogen sulphotransferase (EST) sulphates active oestrogen. The roles of both STS and EST have not been examined in oestrogen-dependent non-small-cell lung cancer (NSCLC). METHODS: We evaluated the immunoreactivity of STS and EST in NSCLC cases using immunohistochemistry. The function of STS and EST was further demonstrated using NSCLC cell lines. RESULTS: The immunoreactivity of STS and EST was detected in 49.5% and 27.8% of NSCLC cases, respectively. The immunoreactivity of STS was significantly higher in female adenocarcinoma cases. The STS-positive NSCLCs were also significantly correlated in an inversed manner with tumour size and cell proliferation and tended to be associated with better clinical outcome. However, the immunoreactivity of EST was significantly correlated with intracellular oestradiol concentration. Results of in vitro analysis demonstrated that oestrone sulphate (E1-S) induced and pregnenolone sulphate (Preg-S) inhibited the proliferation in STS-expressing cell lines. The inhibition by Preg-S was reversed by a specific progesterone receptor blocker. Simultaneous addition of E1-S and Preg-S significantly suppressed the proliferation. CONCLUSION: In NSCLC patients, STS is considered a good prognostic factor. Results of our present study also indicated the benefits of potential progesterone therapy for NSCLC patients.
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spelling pubmed-36294332014-04-16 Steroid sulphatase and oestrogen sulphotransferase in human non-small-cell lung carcinoma Iida, S Kakinuma, H Miki, Y Abe, K Sakurai, M Suzuki, S Niikawa, H Akahira, J Suzuki, T Sasano, H Br J Cancer Clinical Study BACKGROUND: Steroid sulphatase (STS) is one of the steroid-metabolising enzymes involved in desulphating inactive steroid sulphates and oestrogen sulphotransferase (EST) sulphates active oestrogen. The roles of both STS and EST have not been examined in oestrogen-dependent non-small-cell lung cancer (NSCLC). METHODS: We evaluated the immunoreactivity of STS and EST in NSCLC cases using immunohistochemistry. The function of STS and EST was further demonstrated using NSCLC cell lines. RESULTS: The immunoreactivity of STS and EST was detected in 49.5% and 27.8% of NSCLC cases, respectively. The immunoreactivity of STS was significantly higher in female adenocarcinoma cases. The STS-positive NSCLCs were also significantly correlated in an inversed manner with tumour size and cell proliferation and tended to be associated with better clinical outcome. However, the immunoreactivity of EST was significantly correlated with intracellular oestradiol concentration. Results of in vitro analysis demonstrated that oestrone sulphate (E1-S) induced and pregnenolone sulphate (Preg-S) inhibited the proliferation in STS-expressing cell lines. The inhibition by Preg-S was reversed by a specific progesterone receptor blocker. Simultaneous addition of E1-S and Preg-S significantly suppressed the proliferation. CONCLUSION: In NSCLC patients, STS is considered a good prognostic factor. Results of our present study also indicated the benefits of potential progesterone therapy for NSCLC patients. Nature Publishing Group 2013-04-16 2013-03-26 /pmc/articles/PMC3629433/ /pubmed/23531699 http://dx.doi.org/10.1038/bjc.2013.84 Text en Copyright © 2013 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/3.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Clinical Study
Iida, S
Kakinuma, H
Miki, Y
Abe, K
Sakurai, M
Suzuki, S
Niikawa, H
Akahira, J
Suzuki, T
Sasano, H
Steroid sulphatase and oestrogen sulphotransferase in human non-small-cell lung carcinoma
title Steroid sulphatase and oestrogen sulphotransferase in human non-small-cell lung carcinoma
title_full Steroid sulphatase and oestrogen sulphotransferase in human non-small-cell lung carcinoma
title_fullStr Steroid sulphatase and oestrogen sulphotransferase in human non-small-cell lung carcinoma
title_full_unstemmed Steroid sulphatase and oestrogen sulphotransferase in human non-small-cell lung carcinoma
title_short Steroid sulphatase and oestrogen sulphotransferase in human non-small-cell lung carcinoma
title_sort steroid sulphatase and oestrogen sulphotransferase in human non-small-cell lung carcinoma
topic Clinical Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3629433/
https://www.ncbi.nlm.nih.gov/pubmed/23531699
http://dx.doi.org/10.1038/bjc.2013.84
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